Key Product Details

Species Reactivity

Human

Applications

ELISA

Label

Janelia Fluor 549

Antibody Source

Monoclonal Mouse IgG2B Clone # XM207
View all Progesterone Primary Antibodies »

Product Specifications

Immunogen

Hybridoma clone has been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with purified progesterone conjugated with BSA.

Localization

Secreted

Specificity

Progesterone, Ka = 3.0 x 10^9_ M^-1. Not cross-reacting with BSA. Cross-reactivity (%): Progesterone (100); 17-Hydroxyprogesterone (1.0); 11-Hydroxyprogesterone (25); 5-alpha-pregnane-3,20 dionene (10.5); Corticosterone (0.01); Pregnenolone (0.9); Deoxycorticosterone (0.30); Deoxycortisol (0.03); Cortisol (0.002)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Applications for Progesterone Antibody (XM207) [Janelia Fluor® 549]

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Ion exchange chromatography

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Progesterone

Progesterone plays a central role in the reproductive events associated with the establishment and maintenance of pregnancy. Progesterone receptor, a member of the steroid receptor superfamily, mediates the physiologic effects of progesterone. The PGR gene uses separate promoters and translational start sites to produce 2 isoforms, PRA and PRB, which are identical except for an additional 165 amino acids present only in the N terminus of PRB. Although PRA and PRB share several structural domains, they are distinct transcription factors that mediate their own response genes and physiologic effects with little overlap. It is composed of three domains: a modulating N terminal domain, a DNA binding domain and a C terminal steroid binding domain. Progesterone levels 1. men 30-60 pg/0.1ml 2. women pre ovulatory phase: 20-160 pg/0.1ml; ovulatory phase: 1,000-1,700 pg/0.1ml; post ovulatory phase: 1,000-1,700 pg/0.1ml; Pregnant: 16-18 weeks: 300-800 pg/0.1ml; 28-30 weeks: 6,500-14,700 pg/0.1ml; 38-40 weeks: 12,000-19,000 pg/0.1ml.

Alternate Names

4-Pregnen-3,20-dione

Additional Progesterone Products

Product Documents for Progesterone Antibody (XM207) [Janelia Fluor® 549]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Progesterone Antibody (XM207) [Janelia Fluor® 549]



Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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