Mouse Progesterone ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP2-60125

Novus Biologicals
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Key Product Details

Sample Type & Volume Required Per Well

Serum and Plasma (50 - 100 uL)

Sensitivity

0.2 ng/mL

Assay Range

0.3 ng/mL - 10 ng/mL
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Product Specifications

Assay Type

Competitive ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Mouse

Specificity

This assay has high sensitivity and excellent specificity for detection of mouse PROG. No significant cross-reactivity or interference between mouse PROG and analogues was observed.

Description

Assay Length: 1-5 hours

Precision

Intra-Assay Precision (Precision within an assay) %CV 15

Inter-Assay Precision (Precision between assays) %CV 15

Scientific Data Images for Mouse Progesterone ELISA Kit (Colorimetric)

Mouse Progesterone ELISA Kit (Colorimetric)

Mouse Progesterone ELISA Kit (Colorimetric) [NBP2-60125] -

Mouse Progesterone ELISA Kit (Colorimetric) [NBP2-60125] - Standard Curve Reference

Kit Contents for Mouse Progesterone ELISA Kit (Colorimetric)

  • Adhesive Strip (For 96 wells)
  • Antibody
  • Assay plate
  • HRP-conjugate
  • Instruction manual
  • Standard
  • Stop Solution
  • Substrate A
  • Substrate B
  • Wash Buffer (20 x concentrate)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Progesterone

This assay employs the competitive enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with goat-anti-rabbit antibody. Standards or samples are added to the appropriate microtiter plate wells with an antibody specific for PROG and Horseradish Peroxidase (HRP) conjugated PROG. The competitive inhibition reaction is launched between with HRP labeled PROG and unlabeled PROG with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of PROG in the sample. The color development is stopped and the intensity of the color is measured.

Alternate Names

4-Pregnen-3,20-dione

Additional Progesterone Products

Product Documents for Mouse Progesterone ELISA Kit (Colorimetric)

Certificate of Analysis

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Product Specific Notices for Mouse Progesterone ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Citations for Mouse Progesterone ELISA Kit (Colorimetric)

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