Prostatic Acid Phosphatase/ACPP Antibody

Novus Biologicals | Catalog # NBP1-78280

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
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Product Specifications

Immunogen

Peptide with sequence C-NHMKRATQIPSYKK corresponding to internal region according to NP_001127666.1, NP_001090.2.

Epitope

C-NHMKRATQIPSYKK,

Specificity

This antibody is expected to recognize both reported isoforms (NP_001127666.1; NP_001090.2).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Prostatic Acid Phosphatase/ACPP Antibody

Western Blot: Prostatic Acid Phosphatase/ACPP Antibody [NBP1-78280]

Western Blot: Prostatic Acid Phosphatase/ACPP Antibody [NBP1-78280]

Western Blot: Prostatic Acid Phosphatase/ACPP Antibody [NBP1-78280] - Staining of Human Prostate lysate with antibody at 0.01 ug/mL (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: Prostatic Acid Phosphatase/ACPP Antibody [NBP1-78280]

Immunohistochemistry-Paraffin: Prostatic Acid Phosphatase/ACPP Antibody [NBP1-78280]

Immunohistochemistry-Paraffin: Prostatic Acid Phosphatase/ACPP Antibody [NBP1-78280] - Staining of paraffin embedded Human Prostate with antibody at 3.8 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for Prostatic Acid Phosphatase/ACPP Antibody

Application
Recommended Usage

Immunohistochemistry

3 - 5 ug/mL

Immunohistochemistry-Paraffin

3 - 5 ug/mL

Peptide ELISA

Detection limit 1:32000

Western Blot

0.01 - 0.03 ug/mL
Application Notes
WB: Approx. 50 kDa band observed in human prostate lysates (calculated MW of 48.3 kDa band according to NP_001127666.1). IHC-P: Human prostate shows reticulate cytoplasm staining, more intens towards the lumen of the gland.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Prostatic Acid Phosphatase/ACPP

Prostatic Acid Phosphatase encodes an enzyme which catalyzes the conversion of orthophosphoric monoester to alcohol and orthophosphate. It is synthesized under androgen regulation and secreted by the epithelial cells of the prostrate gland.

Alternate Names

ACP3, ACPP

Gene Symbol

ACP3

Additional Prostatic Acid Phosphatase/ACPP Products

Product Documents for Prostatic Acid Phosphatase/ACPP Antibody

Certificate of Analysis

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Product Specific Notices for Prostatic Acid Phosphatase/ACPP Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Citations for Prostatic Acid Phosphatase/ACPP Antibody

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Protocols

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