Proteasome 20S alpha 6 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-33538

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (100%), Chicken (96%), Chimpanzee (100%), Porcine (98%), Rhesus Macaque (100%), Sheep (99%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Proteasome 20S alpha 6. The exact sequence is proprietary.

Reactivity Notes

Xenopus laevis (87%).

Localization

Cytoplasm, Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

27 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Proteasome 20S alpha 6 Antibody - BSA Free

Western Blot: Proteasome 20S alpha 6 Antibody [NBP1-33538]

Western Blot: Proteasome 20S alpha 6 Antibody [NBP1-33538]

Western Blot: Proteasome 20S alpha 6 Antibody [NBP1-33538] - A: 293T B: A431 C: H1299 D: Hela 12% SDS PAGE; antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: Proteasome 20S alpha 6 Antibody [NBP1-33538]

Immunocytochemistry/ Immunofluorescence: Proteasome 20S alpha 6 Antibody [NBP1-33538]

Immunocytochemistry/Immunofluorescence: Proteasome 20S alpha 6 Antibody [NBP1-33538] - Paraformaldehyde-fixed A431, using Proteasome 20S alpha 6 (NBP1-33538) antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: Proteasome 20S alpha 6 Antibody [NBP1-33538]

Immunohistochemistry-Paraffin: Proteasome 20S alpha 6 Antibody [NBP1-33538]

Immunohistochemistry-Paraffin: Proteasome 20S alpha 6 Antibody [NBP1-33538] - Paraffin-embedded mouse liver. Proteasome 20S alpha 6 antibody dilution: 1:500.
Western Blot: Proteasome 20S alpha 6 Antibody [NBP1-33538]

Western Blot: Proteasome 20S alpha 6 Antibody [NBP1-33538]

Western Blot: Proteasome 20S alpha 6 Antibody [NBP1-33538] - A. 50 ug mouse liver lysate/extract 12 % SDS-PAGEProteasome 20S alpha 6 antibody dilution: 1:1000
Western Blot: Proteasome 20S alpha 6 Antibody [NBP1-33538]

Western Blot: Proteasome 20S alpha 6 Antibody [NBP1-33538]

Western Blot: Proteasome 20S alpha 6 Antibody [NBP1-33538] - A. 50 ug rat liver lysate/extract 12 % SDS-PAGE Proteasome 20S alpha 6 antibody dilution: 1:1000
Immunohistochemistry-Paraffin: Proteasome 20S alpha 6 Antibody [NBP1-33538]

Immunohistochemistry-Paraffin: Proteasome 20S alpha 6 Antibody [NBP1-33538]

Immunohistochemistry-Paraffin: Proteasome 20S alpha 6 Antibody [NBP1-33538] - Paraffin-embedded H1299 xenograft, using antibody at 1:100 dilution.
Immunohistochemistry-Paraffin: Proteasome 20S alpha 6 Antibody [NBP1-33538]

Immunohistochemistry-Paraffin: Proteasome 20S alpha 6 Antibody [NBP1-33538]

Immunohistochemistry-Paraffin: Proteasome 20S alpha 6 Antibody [NBP1-33538] - Paraffin-embedded mouse lung. Proteasome 20S alpha 6 antibody dilution: 1:500.
Immunohistochemistry-Paraffin: Proteasome 20S alpha 6 Antibody [NBP1-33538]

Immunohistochemistry-Paraffin: Proteasome 20S alpha 6 Antibody [NBP1-33538]

Immunohistochemistry-Paraffin: Proteasome 20S alpha 6 Antibody [NBP1-33538] - Paraffin-embedded mouse urinary bladder. Proteasome 20S alpha 6 antibody dilution: 1:500.

Applications for Proteasome 20S alpha 6 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris, 0.1M Glycine, 20% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Proteasome 20S alpha 6

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. A pseudogene has been identified on the Y chromosome.

Long Name

Proteasome subunit alpha type-6

Alternate Names

p27K, PROS-27, PROS27, PSMA6

Gene Symbol

PSMA6

Additional Proteasome 20S alpha 6 Products

Product Documents for Proteasome 20S alpha 6 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Proteasome 20S alpha 6 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

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