Recombinant Protein A Cys (C-Term) Protein

Novus Biologicals | Catalog # NBP2-35001

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Key Product Details

Source

E. coli

Tag

Cys (C-Term)

Applications

Bioactivity, SDS-PAGE
View all Protein A Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 297 amino acids corresponding to Protein A with a Cys on the C-terminusSource: E. coli

Uniprot ID: P02976

Amino Acid Sequence: NAAQHDEAQQ NAFYQVLNMP NLNADQRNGF IQSLKDDPSQ SANVLGEAQK LNDSQAPKAD AQQNNFNKDQ QSAFYEILNM PNLNEAQRNG FIQSLKDDPS QSTNVLGEAK KLNESQAPKA DNNFNKEQQN AFYEILNMPN LNEEQRNGFI QSLKDDPSQS ANLLSEAKKL NESQAPKADN KFNKEQQNAF YEILHLPNLN EEQRNGFIQS LKDDPSQSAN LLAEAKKLND AQAPKADNKF NKEQQNAFYE ILHLPNLTEE QRNGFIQSLK DDPSVSKEIL AEAKKLNDAQ APKEEDC

Purity

> 98 % pure by SDS-PAGE and HPLC

Endotoxin Level

Less than 0.1 EU/ug of Protein A as determined by LAL method.

Predicted Molecular Mass

33.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Protein A is a genetically engineered protein containing 5 IgG-binding regions of protein A. Cell wall binding region, cell membrane binding region and albumin binding region have been removed from the recombinant Protein A to ensure the maximum specific IgG binding. The recombinant Protein A is ideal for purification of polyclonal or monoclonal IgG antibodies. Protein A binds to various human, mouse and rat IgG subclasses (e.g., human IgG1, IgG2, IgG4; mouse IgG2a, IgG2b, IgG3; rat IgG2c). It also binds to

Specificity

Protein A binds to various human, mouse and rat IgG subclasses (e.g., human IgG1, IgG2, IgG4; mouse IgG2a, IgG2b, IgG3; rat IgG2c). It also binds to total IgG from rabbit, guinea pig, pig, dog and cat.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Protein A Cys (C-Term) Protein

SDS-PAGE: Recombinant Protein A Cys (C-Term) Protein [NBP2-35001]

SDS-PAGE: Recombinant Protein A Cys (C-Term) Protein [NBP2-35001]

SDS-Page: Protein A Cys (C-Term) Protein [NBP2-35001]
HPLC: Recombinant Protein A Cys (C-Term) Protein [NBP2-35001]

HPLC: Recombinant Protein A Cys (C-Term) Protein [NBP2-35001]

HPLC: Recombinant Protein A Protein [NBP2-35001] - Protein A Cys (C-Term) Protein [NBP2-35001]
HPLC: Recombinant Protein A Cys (C-Term) Protein [NBP2-35001]

HPLC: Recombinant Protein A Cys (C-Term) Protein [NBP2-35001]

HPLC: Recombinant Protein A Protein [NBP2-35001] - Protein A Cys (C-Term) Protein [NBP2-35001]

Formulation, Preparation, and Storage

NBP2-35001
Formulation Lyophilized from additive free solution.
Preservative No Preservative
Concentration Lyoph
Reconstitution Reconstitute with distilled water or saline.
Format Carrier-Free
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Protein A

Protein A is a cell wall component produced by several strains of Staphylococcus aureus that consists of a single polypeptide chain and contains little or no carbohydrate. Recombinant Protein A is produced in E.Coli and functions essentially the same as native Protein A. It consists of 5 IgG-binding domains E-D-A-B-C aligned in series.

Alternate Names

PROA, Protein-A

Gene Symbol

SAOUHSC_00069

Additional Protein A Products

Product Documents for Recombinant Protein A Cys (C-Term) Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Recombinant Protein A Cys (C-Term) Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

Citations for Recombinant Protein A Cys (C-Term) Protein

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FAQs for Recombinant Protein A Cys (C-Term) Protein

Showing  1 - 2 of 2 FAQs Showing All

  • Q: During my master thesis, I am working on a large scale ribosome affinity purification in Trypanosoma brucei. Unfortunately, we have some problems with the present antibody (IgG against protein A, extracted from chicken). Therefore, we are searching a better and more efficient alternative. Ideally it is extracted from guinea pig, rat, mouse or rabbit. Searching suitable antibodies, I found you have some in your range of products. Since we need to work large scale and will need a bigger amount of antibodies the next months, we would like to get the possibility to test the new antibodies first. Therefore I would like to ask for an aliquot for testing. You would really help me finding an good alternative!

    A:

    We unfortunately cannot offer testing samples of our antibodies. I am sorry for any inconvenience. We do have two antibodies to Protein A that meet your host requirements. We fully guarantee our antibodies for all listed species and applications. For any applications and/or species not listed, we offer our Innovators Reward Program. In exchange for a review of your experiment using our product in an untested application or species, we will issue you a credit for the purchase price of the antibody.

  • Q: We ordered the protein A-Cys try to do a conjugation,NBP2-35001-100mg. Do we need to activate the Cys before use? the other protein is activated by SMCC

    A: From the QC data of the product development team, we find protein A-cys only has about 20-30% free sulfhydryl groups while 70-80% sulfhydryl groups are disulfide bonds. So before SMCC activation, we suggest that you treat the sample with reducing reagent such as DTT or beta-ME to release more free sulfhydryl groups.

  • Q: During my master thesis, I am working on a large scale ribosome affinity purification in Trypanosoma brucei. Unfortunately, we have some problems with the present antibody (IgG against protein A, extracted from chicken). Therefore, we are searching a better and more efficient alternative. Ideally it is extracted from guinea pig, rat, mouse or rabbit. Searching suitable antibodies, I found you have some in your range of products. Since we need to work large scale and will need a bigger amount of antibodies the next months, we would like to get the possibility to test the new antibodies first. Therefore I would like to ask for an aliquot for testing. You would really help me finding an good alternative!

    A:

    We unfortunately cannot offer testing samples of our antibodies. I am sorry for any inconvenience. We do have two antibodies to Protein A that meet your host requirements. We fully guarantee our antibodies for all listed species and applications. For any applications and/or species not listed, we offer our Innovators Reward Program. In exchange for a review of your experiment using our product in an untested application or species, we will issue you a credit for the purchase price of the antibody.

  • Q: We ordered the protein A-Cys try to do a conjugation,NBP2-35001-100mg. Do we need to activate the Cys before use? the other protein is activated by SMCC

    A: From the QC data of the product development team, we find protein A-cys only has about 20-30% free sulfhydryl groups while 70-80% sulfhydryl groups are disulfide bonds. So before SMCC activation, we suggest that you treat the sample with reducing reagent such as DTT or beta-ME to release more free sulfhydryl groups.

Showing  1 - 2 of 2 FAQs Showing All
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