Mouse Protein Disulfide Isomerase/P4HB ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP2-81223

Novus Biologicals
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma, cell supernatant, and other biological fluids (100 ul)

Sensitivity

0.09 ng/mL

Assay Range

0.16 - 10 ng/mL
View all Protein Disulfide Isomerase/P4HB ELISA Kits »

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Mouse

Specificity

This kit recognizes Mouse Protein Disulfide Isomerase/P4HB in samples. No significant cross-reactivity or interference between Mouse Protein Disulfide Isomerase/P4HB and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% 5.56%

Inter-Assay Precision (Precision between assays) CV% 5.79%

Recovery for Mouse Protein Disulfide Isomerase/P4HB ELISA Kit (Colorimetric)

Recovery

87-109%

Linearity

Mouse Protein Disulfide Isomerase/P4HB ELISA Kit (Colorimetric)

Scientific Data Images for Mouse Protein Disulfide Isomerase/P4HB ELISA Kit (Colorimetric)

Mouse Protein Disulfide Isomerase/P4HB ELISA Kit (Colorimetric)

ELISA: Mouse Protein Disulfide Isomerase/P4HB ELISA Kit (Colorimetric) [NBP2-81223] -

ELISA: Mouse Protein Disulfide Isomerase/P4HB ELISA Kit (Colorimetric) [NBP2-81223] - Standard Curve Reference

Kit Contents for Mouse Protein Disulfide Isomerase/P4HB ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Protein Disulfide Isomerase/P4HB

P4HB, the beta subunit of prolyl 4-hydroxylase, a highly abundant member of the PDI family. P4HB is a multifunctional enzyme, involved in hydroxylation of prolyl residues in preprocollagen, as well as continual rearrangement of disulfide bonds. Other known functions include its ability to act as a chaperone that inhibits aggregation of misfolded proteins in a concentration-dependent manner, its ability to bind thyroid hormone, its role in both the influx and efflux of S-nitrosothiol-bound nitric oxide, and its function as a subunit of the microsomal triglyceride transfer protein complex.

Long Name

Proline 4-Hydroxylase, Beta Polypeptide

Alternate Names

DSI, ERBA2L, GIT, PDI, PDIA1, PO4DB, PO4HB, PROHB, Thbp

Gene Symbol

P4HB

Additional Protein Disulfide Isomerase/P4HB Products

Product Documents for Mouse Protein Disulfide Isomerase/P4HB ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Mouse Protein Disulfide Isomerase/P4HB ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Related Research Areas

Redox Enzymes

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