Protein O-Fucosyltransferase 1/POFUT1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-76940

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Rat (94%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Protein O-Fucosyltransferase 1/POFUT1 Primary Antibodies »

Product Specifications

Immunogen

Antibody was raised against a 16 amino acid synthetic peptide from near the amino terminus of human POFUT1. The immunogen is located within amino acids 90 - 140 of POFUT1.

Specificity

At least two isoforms of POFUT1 are known to exist. This antibody is predicted to not cross-react with POFUT2.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

44 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Protein O-Fucosyltransferase 1/POFUT1 Antibody - BSA Free

Western Blot: Protein O-Fucosyltransferase 1/POFUT1 AntibodyBSA Free [NBP1-76940]

Western Blot: Protein O-Fucosyltransferase 1/POFUT1 AntibodyBSA Free [NBP1-76940]

Western Blot: Protein O-Fucosyltransferase 1/POFUT1 Antibody [NBP1-76940] - Human heart tissue lysate with POFUT1 antibody at (A) 0.5 and (B) 1 ug/ml.
Protein O-Fucosyltransferase 1/POFUT1 Antibody - BSA Free

Immunohistochemistry: Protein O-Fucosyltransferase 1/POFUT1 Antibody - BSA Free [NBP1-76940] -

Immunohistochemistry: Protein O-Fucosyltransferase 1/POFUT1 Antibody - BSA Free [NBP1-76940] - Immunohistochemistry of Protein O-Fucosyltransferase 1/POFUT1 in human heart tissue with POFUT antibody at 2.5 ug/mL.

Applications for Protein O-Fucosyltransferase 1/POFUT1 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunohistochemistry

2.5 ug/ml

Immunohistochemistry-Paraffin

2.5 ug/ml

Western Blot

0.5-1 ug/ml

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Protein O-Fucosyltransferase 1/POFUT1

POFUT1, an endoplasmic reticulum-residing member of the glycosyltransferase O-Fuc family, adds O-fucose through an O-glycosidic linkage to conserved serine or threonines in epidermal growth factor-like repeats of several cell surface and secreted proteins. Unlike its homolog POFUT2, POFUT1 can also catalyze the transfer of fucose to thrombospondin type 1 repeats. Many of the substrates of POFUT1 are involved in ligand-induced receptor signaling. One such protein is Notch; mouse ES cells lacking POFUT have normal levels of Notch receptors at the cell surface, but these receptors do not bind Notch ligands or exhibit Notch signaling. At least two isoforms of POFUT1 are known to exist. This antibody is predicted to not cross-react with POFUT2.

Long Name

Protein O‑fucosyltransferase 1/POFUT1

Alternate Names

FucT-1, FUT12, O-Fuc-T, O-FUT, POFUT1, Protein OFucosyltransferase 1

Entrez Gene IDs

23509 (Human); 140484 (Mouse)

Gene Symbol

POFUT1

UniProt

Q9H488 (Human)

Additional Protein O-Fucosyltransferase 1/POFUT1 Products

Product Documents for Protein O-Fucosyltransferase 1/POFUT1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Protein O-Fucosyltransferase 1/POFUT1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

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Associated Pathways

Notch Signaling Pathways Notch Signaling Pathway Thumbnail