Protocadherin-8 Antibody (6A8) - Azide and BSA Free

Novus Biologicals | Catalog # H00005100-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Sandwich ELISA, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 6A8

Format

Azide and BSA Free
View all Protocadherin-8 Primary Antibodies »

Product Specifications

Immunogen

PCDH8 (NP_002581, 32 a.a. ~ 120 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. VRYSTFEEDAPGTVIGTLAEDLHMKVSGDTSFRLMKQFNSSLLRVREGDGQLTVGDAGLDRERLCGQAPQCVLAFDVVSFSQEQFRLVH

Specificity

PCDH8 - protocadherin 8

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Protocadherin-8 Antibody (6A8) - Azide and BSA Free

Western Blot: Protocadherin-8 Antibody (6A8) [H00005100-M01]

Western Blot: Protocadherin-8 Antibody (6A8) [H00005100-M01]

Western Blot: Protocadherin-8 Antibody (6A8) [H00005100-M01] - Analysis of PCDH8 expression in transfected 293T cell line by PCDH8 monoclonal antibody (M01), clone 6A8.Lane 1: PCDH8 transfected lysate(113 KDa).Lane 2: Non-transfected lysate.
Immunohistochemistry-Paraffin: Protocadherin-8 Antibody (6A8) [H00005100-M01]

Immunohistochemistry-Paraffin: Protocadherin-8 Antibody (6A8) [H00005100-M01]

Immunohistochemistry-Paraffin: Protocadherin-8 Antibody (6A8) [H00005100-M01] - Analysis of monoclonal antibody to PCDH8 on formalin-fixed paraffin-embedded human tonsil. Antibody concentration 3 ug/ml.
Western Blot: Protocadherin-8 Antibody (6A8) [H00005100-M01]

Western Blot: Protocadherin-8 Antibody (6A8) [H00005100-M01]

Western Blot: Protocadherin-8 Antibody (6A8) [H00005100-M01] - Analysis of PCDH8 over-expressed 293 cell line, cotransfected with PCDH8 Validated Chimera RNAi ( Cat # H00005100-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with PCDH8 monoclonal antibody (M01), clone 6A8 (Cat # H00005100-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western Blot: Protocadherin-8 Antibody (6A8) [H00005100-M01]

Western Blot: Protocadherin-8 Antibody (6A8) [H00005100-M01]

Western Blot: Protocadherin-8 Antibody (6A8) [H00005100-M01] - PCDH8 monoclonal antibody (M01), clone 6A8 Analysis of PCDH8 expression in COLO 320 HSR.
Sandwich ELISA: Protocadherin-8 Antibody (6A8) [H00005100-M01] - Detection limit for recombinant GST tagged PCDH8 is approximately 0.1ng/ml as a capture antibody.

Applications for Protocadherin-8 Antibody (6A8) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IHC-P, RNAi Validation and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Protocadherin-8

This gene belongs to the protocadherin gene family, a subfamily of the cadherin superfamily. The gene encodes an integral membrane protein that is thought to function in cell adhesion in a CNS-specific manner. Unlike classical cadherins, which are generally encoded by 15-17 exons, this gene includes only 3 exons. Notable is the large first exon encoding the extracellular region, including 6 cadherin domains and a transmembrane region. Alternative splicing yields isoforms with unique cytoplasmic tails.

Alternate Names

Arcadlin, PAPC, PCDH8, Protocadherin8

Entrez Gene IDs

5100 (Human)

Gene Symbol

PCDH8

OMIM

603580 (Human)

UniProt

NP_002581 (Human)

Additional Protocadherin-8 Products

Product Documents for Protocadherin-8 Antibody (6A8) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Protocadherin-8 Antibody (6A8) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cadherin Superfamily

Citations for Protocadherin-8 Antibody (6A8) - Azide and BSA Free

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Protocols

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FAQs

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