Recombinant Human PSMB5 His Protein

Novus Biologicals | Catalog # NBP1-50934

Novus Biologicals
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Key Product Details

Source

E. coli

Tag

His

Applications

SDS-PAGE
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Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 60-263 of Human PSMB5

Source: E.coli

Amino Acid Sequence: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMTTT LAFKFRHGVI VAADSRATAG AYIASQTVKK VIEINPYLLG TMAGGAADCS FWERLLARQC RIYELRNKER ISVAAASKLL ANMVYQYKGM GLSMGTMICG WDKRGPGLYY VDSEGNRISG ATFSVGSGSV YAYGVMDRGY SYDLEVEQAY DLARRAIYQA TYRDAYSGGA VNLYHVREDG WIRVSSDNVA DLHEKYSGST P

Purity

>90%, by SDS-PAGE

Predicted Molecular Mass

26.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human PSMB5 His Protein

SDS-PAGE: Recombinant Human PSMB5 His Protein [NBP1-50934]

SDS-PAGE: Recombinant Human PSMB5 His Protein [NBP1-50934]

SDS-Page: Recombinant Human PSMB5 Protein [NBP1-50934] - 15 % SDS-PAGE (3ug)

Formulation, Preparation, and Storage

NBP1-50934
Formulation 20 mM Tris-HCl buffer (pH 8.5), 50% glycerol, 1mM DTT, 0.15 M NaCl
Preservative No Preservative
Concentration 0.25 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PSMB5

PSMB5 is a member of the proteasome B-type family that is a 20S core beta subunit in the proteasome. This catalytic subunit is not present in the immunoproteasome and is replaced by catalytic subunit 3i (proteasome beta 8 subunit). PSMB5 has an ATP dependent proteolytic activity and involved in an ATP/ubiquitin dependent non lysosomal proteolytic pathway. Recombinant human PSMB5 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Long Name

Proteasome subunit beta type-5

Alternate Names

LMPX, MB1, Proteasome chain 6

Gene Symbol

PSMB5

Additional PSMB5 Products

Product Documents for Recombinant Human PSMB5 His Protein

Certificate of Analysis

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Product Specific Notices for Recombinant Human PSMB5 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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