Key Product Details

Species Reactivity

Human, Mouse, Rat, Canine

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI9F3

Format

Azide and BSA Free
View all PSMC3 Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human PSMC3(NP_002795) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

49.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PSMC3 Antibody (OTI9F3) - Azide and BSA Free

Western Blot: PSMC3 Antibody (OTI9F3)Azide and BSA Free [NBP2-73696]

Western Blot: PSMC3 Antibody (OTI9F3)Azide and BSA Free [NBP2-73696]

Western Blot: PSMC3 Antibody (OTI9F3) - Azide and BSA Free [NBP2-73696] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PSMC3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PSMC3.
Immunocytochemistry/ Immunofluorescence: PSMC3 Antibody (OTI9F3) - Azide and BSA Free [NBP2-73696]

Immunocytochemistry/ Immunofluorescence: PSMC3 Antibody (OTI9F3) - Azide and BSA Free [NBP2-73696]

Immunocytochemistry/Immunofluorescence: PSMC3 Antibody (OTI9F3) - Azide and BSA Free [NBP2-73696] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY PSMC3.
Immunohistochemistry: PSMC3 Antibody (OTI9F3) - Azide and BSA Free [NBP2-73696]

Immunohistochemistry: PSMC3 Antibody (OTI9F3) - Azide and BSA Free [NBP2-73696]

Immunohistochemistry: PSMC3 Antibody (OTI9F3) - Azide and BSA Free [NBP2-73696] - Staining of paraffin-embedded prostate tissue using anti-PSMC3 mouse monoclonal antibody.
Flow Cytometry: PSMC3 Antibody (OTI9F3) - Azide and BSA Free [NBP2-73696]

Flow Cytometry: PSMC3 Antibody (OTI9F3) - Azide and BSA Free [NBP2-73696]

Flow Cytometry: PSMC3 Antibody (OTI9F3) - Azide and BSA Free [NBP2-73696] - Analysis of Jurkat cells, using anti-PSMC3 antibody, (Red) compared to a nonspecific negative control antibody (Blue).
Western Blot: PSMC3 Antibody (OTI9F3)Azide and BSA Free [NBP2-73696]

Western Blot: PSMC3 Antibody (OTI9F3)Azide and BSA Free [NBP2-73696]

Western Blot: PSMC3 Antibody (OTI9F3) - Azide and BSA Free [NBP2-73696] - Analysis of extracts (35ug) from 9 different cell lines by using anti-PSMC3 monoclonal antibody.
Immunohistochemistry: PSMC3 Antibody (OTI9F3) - Azide and BSA Free [NBP2-73696]

Immunohistochemistry: PSMC3 Antibody (OTI9F3) - Azide and BSA Free [NBP2-73696]

Immunohistochemistry: PSMC3 Antibody (OTI9F3) - Azide and BSA Free [NBP2-73696] - Staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-PSMC3 mouse monoclonal antibody.
Immunohistochemistry: PSMC3 Antibody (OTI9F3) - Azide and BSA Free [NBP2-73696]

Immunohistochemistry: PSMC3 Antibody (OTI9F3) - Azide and BSA Free [NBP2-73696]

Immunohistochemistry: PSMC3 Antibody (OTI9F3) - Azide and BSA Free [NBP2-73696] - Staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-PSMC3mouse monoclonal antibody.
Immunohistochemistry: PSMC3 Antibody (OTI9F3) - Azide and BSA Free [NBP2-73696]

Immunohistochemistry: PSMC3 Antibody (OTI9F3) - Azide and BSA Free [NBP2-73696]

Immunohistochemistry: PSMC3 Antibody (OTI9F3) - Azide and BSA Free [NBP2-73696] - Staining of paraffin-embedded bladder tissue using anti-PSMC3 mouse monoclonal antibody.
Immunohistochemistry: PSMC3 Antibody (OTI9F3) - Azide and BSA Free [NBP2-73696]

Immunohistochemistry: PSMC3 Antibody (OTI9F3) - Azide and BSA Free [NBP2-73696]

Immunohistochemistry: PSMC3 Antibody (OTI9F3) - Azide and BSA Free [NBP2-73696] - Staining of paraffin-embedded Carcinoma of bladder tissue using anti-PSMC3 mouse monoclonal antibody.
Immunohistochemistry: PSMC3 Antibody (OTI9F3) - Azide and BSA Free [NBP2-73696]

Immunohistochemistry: PSMC3 Antibody (OTI9F3) - Azide and BSA Free [NBP2-73696]

Immunohistochemistry: PSMC3 Antibody (OTI9F3) - Azide and BSA Free [NBP2-73696] - Staining of paraffin-embedded colon tissue using anti-PSMC3 mouse monoclonal antibody.

Applications for PSMC3 Antibody (OTI9F3) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:50

Western Blot

1:2000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PSMC3

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the ATPase subunits, a member of the triple-A family of ATPases that have chaperone-like activity. This subunit may compete with PSMC2 for binding to the HIV tat protein to regulate the interaction between the viral protein and the transcription complex. A pseudogene has been identified on chromosome 9. [provided by RefSeq]

Alternate Names

human immunodeficiency virus tat transactivator binding protein-1, MGC8487, proteasome (prosome, macropain) 26S subunit, ATPase, 3,26S proteasome AAA-ATPase subunit RPT5, Proteasome 26S subunit ATPase 3, Proteasome subunit P50, TBP-126S protease regulatory subunit 6A, TBP1Tat-binding protein 1

Gene Symbol

PSMC3

Additional PSMC3 Products

Product Documents for PSMC3 Antibody (OTI9F3) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for PSMC3 Antibody (OTI9F3) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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