PTTG1 Antibody

Novus Biologicals | Catalog # NBP2-69985

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all PTTG1 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-ILDEERELEKLFQ, from the internal region of the protein sequence according to NP_004210.1.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for PTTG1 Antibody

Immunocytochemistry/ Immunofluorescence: PTTG1 Antibody [NBP2-69985]

Immunocytochemistry/ Immunofluorescence: PTTG1 Antibody [NBP2-69985]

Immunocytochemistry/Immunofluorescence: PTTG1/2/3 Antibody [NBP2-69985] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining.
Immunohistochemistry-Paraffin: PTTG1 Antibody [NBP2-69985]

Immunohistochemistry-Paraffin: PTTG1 Antibody [NBP2-69985]

Immunohistochemistry-Paraffin: PTTG1/2/3 Antibody [NBP2-69985] - (2.5ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Flow Cytometry: PTTG1 Antibody [NBP2-69985]

Flow Cytometry: PTTG1 Antibody [NBP2-69985]

Flow Cytometry: PTTG1/2/3 Antibody [NBP2-69985] - Analysis of paraformaldehyde fixed Jurkat cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Immunocytochemistry/ Immunofluorescence: PTTG1 Antibody [NBP2-69985]

Immunocytochemistry/ Immunofluorescence: PTTG1 Antibody [NBP2-69985]

Immunocytochemistry/Immunofluorescence: PTTG1/2/3 Antibody [NBP2-69985] - Analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining.
Immunohistochemistry-Paraffin: PTTG1 Antibody [NBP2-69985]

Immunohistochemistry-Paraffin: PTTG1 Antibody [NBP2-69985]

Immunohistochemistry-Paraffin: PTTG1/2/3 Antibody [NBP2-69985] - (2.5ug/ml) staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for PTTG1 Antibody

Application
Recommended Usage

Flow Cytometry

1:10-1:1000

Immunocytochemistry/ Immunofluorescence

1:10 - 1:500

Immunohistochemistry

1:10 - 1:500

Peptide ELISA

1:100-1:2000

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

TBS (pH 7.3), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PTTG1

The product of the oncogene PTTG, pituitary tumor transforming gene, is a human homolog of the anaphase-inhibitor vertebrate protein, securin. PTTG is a 22 kDa protein that contains a basic amino-terminal domain and an acidic carboxy-terminal domain, which acts as a transactivation domain when fused to a heterologous DNA binding domain. Human PTTG is overexpressed in Jurkat and is also detected in human thymus, testis, and placenta. PTTG is mainly expressed in the cytoplasm and is also partially localized to the nucleus. Vertebrate PTTG regulates the separin Esp1, which promotes chromatid separation, to overcome the cohesive forces that hold sister chromatids together. This regulatory function of PTTG suggests that defective regulation of cohesion may contribute to cancer by promoting chromosome instability. Although vertebrate PTTG shares cell-cycle functions with its yeast securin counterparts Pds1p and Cut2, none share sequence homology.

Alternate Names

EAP1MGC126883, Esp1-associated protein, ESP1-associated protein 1, hPTTG, pituitary tumor-transforming 1, Pituitary tumor-transforming gene 1 protein, PTTGMGC138276, securin, Tumor-transforming protein 1, TUTR1HPTTG

Gene Symbol

PTTG1

Additional PTTG1 Products

Product Documents for PTTG1 Antibody

Certificate of Analysis

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Product Specific Notices for PTTG1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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