Puromycin dihydrochloride

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Puromycin dihydrochloride | CAS No. 58-58-2 | DNA, RNA and Protein Synthesis Inhibitors
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Description: Protein synthesis inhibitor

Chemical Name: 3'-[α-Amino-p-methoxyhydrocinnamamido]-3'-deoxy-N,N-dimethyladenosine dihydrochloride

Purity: ≥98%

Product Details
Citations (8)
Reviews (1)

Biological Activity

Puromycin dihydrochloride is a protein synthesis inhibitor; leads to the premature release of polypeptide chains as polypeptidyl purine derivatives. Analog of the 3' end of aminoacyl-tRNA. Aminonucleoside antibiotic. Inhibits translation in both in vitro and in vivo systems. Also inhibits the transport of proteins into the mitochondria in vitro.

Technical Data

Soluble to 100 mM in water and to 100 mM in DMSO
Store at -20°C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.

Background References

  1. Puromycin inhibits protein import into mitochondria by interfering with an intramitochondrial ATP-dependent reaction.
    Price and Verner
    Biochim.Biophys.Acta., 1993;1150:89
  2. Mechanism of puromycin action: fate of ribosomes after release of nascent polypeptide chains from polysomes.
    Azzam and Algranati
    Proc.Nat.Acad.Sci., 1973;70:3866

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Puromycin maintains stable expression of recombinant hRyR2 in HEK293 cells.
By Niroj Shrestha on 01/20/2019
Application: Species: Human

Puromycin (5 mcg/ml) along with geneticin (250 mcg/ml) was used in DMEM containing 10% FBS, 4 mM glutamine and 10 mM HEPES to maintain the stable expression of hRyR2-IRES-GFP in HEK293 cell culture. The stable expression of hRyR2 in these cells was confirmed by cytosolic GFP fluorescence and 10 mM caffeine-evoked cytosolic Ca2+ peak, measured by Fura2 ratiometric dye, as shown in figure.

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