Chemical Name: 3'-[α-Amino-p-methoxyhydrocinnamamido]-3'-deoxy-N,N-dimethyladenosine dihydrochloride
Biological ActivityProtein synthesis inhibitor; leads to the premature release of polypeptide chains as polypeptidyl purine derivatives. Analog of the 3' end of aminoacyl-tRNA. Aminonucleoside antibiotic. Inhibits translation in both in vitro and in vivo systems. Also inhibits the transport of proteins into the mitochondria in vitro.
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Puromycin inhibits protein import into mitochondria by interfering with an intramitochondrial ATP-dependent reaction.
Price and Verner
Mechanism of puromycin action: fate of ribosomes after release of nascent polypeptide chains from polysomes.
Azzam and Algranati
Citations for Puromycin dihydrochloride
The citations listed below are publications that use Tocris products. Selected citations for Puromycin dihydrochloride include:
8 Citations: Showing 1 - 8
SIGMAR1/Sigma-1 receptor ablation impairs autophagosome clearance.
Authors: Yang Et al.
Synergistic antitumor effects of 9.2.27-PE38KDEL and ABT-737 in primary and metastatic brain tumors.
Authors: Yu Et al.
PLoS One 2019;14:e0210608
AGR2 promotes the proliferation, migration and regulates epithelial-mesenchymal transition in salivary adenoid cystic carcinoma.
Authors: Ma Et al.
Am J Transl Res. 2017;9:507
WNT/β-catenin signaling mediates human neural crest induction via a pre-neural border intermediate.
Authors: Leung Et al.
Reduced Levels of Proteasome Products in a Mouse Striatal Cell Model of Huntington's Disease.
Authors: Dasgupta Et al.
PLoS One 2015;10:e0145333
All-Trans Retinoic Acid-Induced Deficiency of the Wnt/β-Catenin Pathway Enhances Hepatic Carcinoma Stem Cell Differentiation.
Authors: Zhu Et al.
J Neurosci 2015;10:e0143255
Proteasome inhibitors alter levels of intracellular peptides in HEK293T and SH-SY5Y cells.
Authors: Dasgupta Et al.
PLoS One 2014;9:e103604
Mitotic phosphorylation of eukaryotic initiation factor 4G1 (eIF4G1) at Ser1232 by Cdk1:cyclin B inhibits eIF4A helicase complex binding with RNA.
Authors: Dobrikov Et al.
Mol Cell Biol 2014;34:439
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Puromycin (5 mcg/ml) along with geneticin (250 mcg/ml) was used in DMEM containing 10% FBS, 4 mM glutamine and 10 mM HEPES to maintain the stable expression of hRyR2-IRES-GFP in HEK293 cell culture. The stable expression of hRyR2 in these cells was confirmed by cytosolic GFP fluorescence and 10 mM caffeine-evoked cytosolic Ca2+ peak, measured by Fura2 ratiometric dye, as shown in figure.