Puromycin dihydrochloride

Tocris Bioscience | Catalog # 4089

Protein synthesis inhibitor
Tocris Bioscience

Key Product Details

Description

Protein synthesis inhibitor
View all DNA, RNA and Protein Synthesis Inhibitors »

Product Description

Puromycin dihydrochloride is a protein synthesis inhibitor; leads to the premature release of polypeptide chains as polypeptidyl purine derivatives. Analog of the 3' end of aminoacyl-tRNA. Aminonucleoside antibiotic. Inhibits translation in both in vitro and in vivo systems. Also inhibits the transport of proteins into the mitochondria in vitro.

Product Specifications for Puromycin dihydrochloride

Molecular Weight

544.43

Formula

C22H29N7O5.2HCl

Storage

Store at -20°C

Purity

≥98% (HPLC)

Chemical Name

3'-[α-Amino-p-methoxyhydrocinnamamido]-3'-deoxy-N,N-dimethyladenosine dihydrochloride

CAS Number

58-58-2

PubChem ID

443311

InChI Key

MKSVFGKWZLUTTO-FZFAUISWSA-N

SMILES

O[C@@H]1[C@H](NC([C@@H](N)CC4=CC=C(OC)C=C4)=O)[C@@H](CO)O[C@H]1N3C2=NC=NC(N(C)C)=C2N=C3.Cl.Cl

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
water 54.44 100
DMSO 54.44 100

Preparing Stock Solutions for Puromycin dihydrochloride

The following data is based on the product molecular weight 544.43.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.84 mL 9.18 mL 18.37 mL
5 mM 0.37 mL 1.84 mL 3.67 mL
10 mM 0.18 mL 0.92 mL 1.84 mL
50 mM 0.04 mL 0.18 mL 0.37 mL

Calculators

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Background References

References are publications that support the biological activity of the product. See our Citations tab to view 32 publications citing the usage of this product.

Product Documents for Puromycin dihydrochloride

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Puromycin dihydrochloride

For research use only

Citations for Puromycin dihydrochloride

Customer Reviews for Puromycin dihydrochloride (1)

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  • Puromycin maintains stable expression of recombinant hRyR2 in HEK293 cells.
    Name: Niroj Shrestha
    Species: Human
    Assay Type: In Vitro
    Cell Line/Tissue: HEK293
    Verified Customer | Posted 01/20/2019
    Puromycin should be stored in aliquots at -20 degree C.
    Puromycin (5 mcg/ml) along with geneticin (250 mcg/ml) was used in DMEM containing 10% FBS, 4 mM glutamine and 10 mM HEPES to maintain the stable expression of hRyR2-IRES-GFP in HEK293 cell culture. The stable expression of hRyR2 in these cells was confirmed by cytosolic GFP fluorescence and 10 mM caffeine-evoked cytosolic Ca2+ peak, measured by Fura2 ratiometric dye, as shown in figure.
    Puromycin dihydrochloride 4089

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