Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody [Janelia Fluor® 669]
Novus Biologicals | Catalog # NB100-2384JF669
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Predicted:
Bovine (100%), Chicken (92%), Porcine (91%), Primate (100%), Rat (100%), Zebrafish (91%). Backed by our 100% Guarantee.
Applications
Western Blot, Immunoblotting
Label
Janelia Fluor 669
Antibody Source
Polyclonal Rabbit IgG
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Product Specifications
Immunogen
A synthetic peptide made to an internal portion of the human PDK1 protein sequence (between residues 300-400). [Swiss-Prot Q15118]
Reactivity Notes
Mouse reactivity reported in scientific literature (PMID: 25925251).
Localization
Mitochondrion
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.
For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
Applications for Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody [Janelia Fluor® 669]
Application
Recommended Usage
Immunoblotting
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Recommended applications are based on validated applications from the unconjugated base product NB100-2384. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: Pyruvate Dehydrogenase Kinase 1/PDK1
PDK1 antibodies are useful tools for glucose metabolism research and mitochondrial metabolism studies.
Alternate Names
EC 2.7.11, EC 2.7.11.2, mitochondrial pyruvate dehydrogenase, lipoamide, kinase isoenzyme 1, PDH kinase 1, PDK1, PDK-1, PKB kinase, Pyruvate dehydrogenase kinase isoform 1, pyruvate dehydrogenase kinase, isoenzyme 1, pyruvate dehydrogenase kinase, isozyme 1, Pyruvate dehydrogenase lipoamide kinase isozyme 1, mitochondrial
Gene Symbol
PDK1
Additional Pyruvate Dehydrogenase Kinase 1/PDK1 Products
Product Documents for Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody [Janelia Fluor® 669]
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody [Janelia Fluor® 669]
Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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