QA1b Antibody (6A8.6F10.1A6) [Allophycocyanin]

Novus Biologicals | Catalog # NBP2-26649APC

Novus Biologicals

Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot, Neutralization, Flow Cytometry, Immunoprecipitation

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 6A8.6F10.1A6
View all QA1b Primary Antibodies »

Product Specifications

Immunogen

Animals were immunized with a synthetic peptide from the alpha-2 domain of Qa-1b. Qa-1 is a Class Ib molecule recognized by cytotoxic T cells and natural killer cells. Spleen cells were fused with P3X63Ag8.653 myeloma cells.The antibody reacts with the Qa-1b Class Ib molecule.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for QA1b Antibody (6A8.6F10.1A6) [Allophycocyanin]

Flow Cytometry: QA1b Antibody (6A8.6F10.1A6) [Allophycocyanin] [NBP2-26649APC] - Experimental autoimmune encephalomyelitis was induced in C57BL6/J mice, and cells from central nervous system (brain+spinal cord) were isolated when the animals were at the peak of the disease. Cells were stained for QA1b, CD8, CD4, TNF, IFNg, IL-17, plus for viability to exclude dead cells. Viable, CD8+ cells were gated and QA1b staining is showed in control cells, non-stained for QA1b (blue), and in QA1b-stained cells (red). Image from verified customer review/

Applications for QA1b Antibody (6A8.6F10.1A6) [Allophycocyanin]

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Neutralization

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 4 using NBP2-26649APC in the following applications:

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer
Spectra Viewer

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: QA1b

Alternate Names

H2-Q1, H2-T23

Gene Symbol

QA1b

Additional QA1b Products

Product Documents for QA1b Antibody (6A8.6F10.1A6) [Allophycocyanin]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for QA1b Antibody (6A8.6F10.1A6) [Allophycocyanin]

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for QA1b Antibody (6A8.6F10.1A6) [Allophycocyanin] (1)

4 out of 5
1 Customer Rating
5 Stars
0%
4 Stars
100%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used QA1b Antibody (6A8.6F10.1A6) [Allophycocyanin]?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 1 of 1 review Showing All
Filter By:
Clear Filters X
  • QA1b Antibody (6A8.6F10.1A6) [Allophycocyanin]
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: brain and spinal cord
    Species: Mouse
    Verified Customer | Posted 02/21/2019
    CNS cells of EAE mice stained for viable cells, QA1b, CD8. CD8+, QA1b non-stained cells (blue) and CD8+, QA1b-stained cells (red).
    Experimental autoimmune encephalomyelitis was induced in C57BL6/J mice, and cells from central nervous system (brain+spinal cord) were isolated when the animals were at the peak of the disease. Cells were stained for QA1b, CD8, CD4, TNF, IFNg, IL-17, plus for viability to exclude dead cells. Viable, CD8+ cells were gated and QA1b staining is showed in control cells, non-stained for QA1b (blue histogram), and in QA1b-stained cells (red histogram).
    QA1b Antibody (6A8.6F10.1A6) [Allophycocyanin] NBP2-26649APC

There are no reviews that match your criteria.

Showing  1 - 1 of 1 review Showing All

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies