Quantikine Immunoassay Control Set 848 Human Neuropilin-1

R&D Systems | Catalog # QC187

For use with catalog number DNRP10
R&D Systems
Discontinued Product
QC187 has been discontinued. An alternative/replacement product is available: QC187B. View all Neuropilin-1 products.

Key Product Details

Species

Human

Applications

Control, ELISA

Product Summary for Quantikine Immunoassay Control Set 848 Human Neuropilin-1

INTENDED USE & DESCRIPTION

For use as quantitative controls for the determination of cytokine concentrations in biological fluids. Concentrations have been assigned using R&D Systems’ Quantikine® kits. Controls are prepared in diluted porcine serum with preservatives. They contain recombinant human cytokines at low, medium and high concentrations. Controls are supplied lyophilized.

STORAGE & STABILITY

Unreconstituted Controls should be stored at 2-8 °C and are stable for at least 6 months from date of receipt. Depending on the analyte of interest, reconstituted controls may be stable when stored at < -20 °C. Users should evaluate the frozen stability of the controls in their application or discard after use.

REAGENT PREPARATION

Reconstitute each vial with the volume of deionized or distilled water indicated on the product datasheet.

PROCEDURE & EXPECTED VALUES

Controls should be assayed in the same manner as unknown specimens.

The acceptable ranges for the analytes in these controls are printed on the product datasheet. Due to possible variations in techniques and methodologies, it is recommended that each laboratory determine its own target range. Laboratories using other test systems should establish their own acceptable ranges as these assays may produce different values.

TECHNICAL HINTS & LIMITATIONS OF THE PROCEDURE 

• The ranges were determined using R&D Systems’ Quantikine kits. If expected values are not obtained, verify that the lot numbers on the vials correspond with the lot numbers listed above and the correct volume of deionized or distilled water was used for reconstitution of the controls. 

• The results obtained with these controls depend upon several factors associated with methods and instrumentation. Test systems other than those supplied by R&D Systems may result in values that differ from those printed on this product datasheet.

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Formulation, Preparation, and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Neuropilin-1

Neuropilin-1 (NRP1) is a transmembrane glycoprotein that regulates axon guidance and angiogenesis. Distinct Neuropilin-1 domains mediate its binding to heparin, the heparin-binding forms of VEGF and PlGF (PlGF2), and Semaphorin 3. Neuropilin-1 can form homo- and hetero-oligomers with Neuropilin-2. Neuropilins show partially overlapping expression in neuronal and endothelial cells during development. They mediate axon repulsion on sensory and sympathetic neurons through binding to Class III Semaphorins, Plexin A3, and Plexin A4.  They also associate with VEGF R2 and function as coreceptors for VEGF. NRP1 is preferentially expressed in developing or remodeling arteries, epithelial cells, and carcinomas, while Neuropilin-2 is primarily found in veins and lymphatics. Neuroplin-1 is additionally expressed on dendritic cells where it mediates DC-induced T cell proliferation. NRP1 also plays a role in SARS-CoV-2 infection in humans, the virus responsible for COVID-19. The Spike protein of SARS-CoV-2 can bind to the b1 domain of Neuropilin-1 on the cell surface initiating viral entry into the host cell. Blocking the NRP1-Spike interaction reduces viral infectivity in vitro making it a potential therapeutic target. 

Alternate Names

BDCA-4, CD304, Neuropilin1, NRP1

Entrez Gene IDs

8829 (Human); 18186 (Mouse); 246331 (Rat); 100286859 (Porcine)

Gene Symbol

NRP1

Additional Neuropilin-1 Products

Product Documents for Quantikine Immunoassay Control Set 848 Human Neuropilin-1

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Quantikine Immunoassay Control Set 848 Human Neuropilin-1

For research use only

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