QuikChIP Kit (ChIP)

Novus Biologicals | Catalog # NBP2-29902

Novus Biologicals
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Key Product Details

Species

Human, Mouse, Bacteria

Applications

Chromatin Immunoprecipitation, Immunoprecipitation

Kit Type

Kit (ChIP)

Product Summary for QuikChIP Kit (ChIP)

The QuikChIP(TM) Kit contains components for 25 ChIP assays optimized for use with transcriptionally active chromatin in adherent or suspension mammalian cells. The protocol is optimized for use with one 10 cm plate containing 5 x 10^6 cells per preparation. Each ChIP assay requires approximately 1 x 10^6 cells. Thus, a 10 cm plate (one preparation) of cells will provide DNA sufficient for five ChIP assays.

Features:
  • Quick and simple.
  • Optimized ready-to-use buffers and protocol are included.
  • Can be used for both histone and non-histone proteins.
  • Reagents for 25 ChIP assays and sheared DNA preparations.

    • Product Specifications

    Applications

    Immunoprecipitation (reported in scientific literature (PMID 21957289))

    Reactivity Notes

    Mouse reactivity reported in scientific literature (PMID: 23709694). Bacteria reactivity reported in scientific literature (PMID: 24363348). Human reactivity reported in scientific literature (PMID: 21086175).

    Scientific Data Images for QuikChIP Kit (ChIP)

    Chromatin Immunoprecipitation: Quick ChIP Kit [NBP2-29902] - Human MCF-7 cells were fixed for 10 minutes with 1% formaldehyde. The cells were lysed and the chromatin sonicated (200-1000 bp fragments). Chromatin was immunoprecipitated using DNMT3b mAb, (Lane 2) and a no antibody control (Lane 3). The PCR amplification was performed with primers specific to the progesterone receptor (PR) promoter region as follows: 94C 5 min, 94C 30 sec, 59C 30 sec 40 cycles, 72C 30 sec, 72C 10 min. Following PCR, 20 ul of each sample was analyzed on a 2% agarose gel and visualized by UV-illumination following ethidium bromide staining. PCR product was observed in the DNMT3b mAb ChIP (Lane 2) and not in the control (Lane 3).
    Quick ChIP Kit [NBP2-29902]

    Kit Contents for QuikChIP Kit (ChIP)

    • 0.5M EDTA (250 uL)
    • 100X PIC (2 x 0.75 mL)
    • 100X PMSF (2 x 0.75 mL)
    • 10X Glycine (25 mL)
    • 10X PBS (55 mL)
    • 1M NaHCO3 (1.25 mL)
    • 1M Tris-HCl pH 6.5 (500 uL)
    • 20% SDS (625 uL)
    • 5M NaCl (500 uL)
    • ChIP Dilution Buffer (50 mL)
    • Proteinase K at 10 mg/mL (60 uL)
    • RNase A at 10 mg/mL (60 uL)
    • Salmon Sperm DNA/Protein A/G Agarose (2 x 1.75 mL)
    • SDS Lysis Buffer (25 mL)
    • Wash Buffer A (25 mL)
    • Wash Buffer B (25 mL)
    • Wash Buffer C (25 mL)
    • Wash Buffer D (25 mL)

    Formulation, Preparation, and Storage

    Concentration

    Concentration is not relevant for this product. Please see the protocols for proper use of this product.

    Shipping

    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

    Storage

    Storage of components varies. See protocol for specific instructions.

    Background: QuikChIP

    Chromatin Immunoprecipitation (ChIP) is a powerful method used to identify regions of the genome associated with specific proteins. These associations are crucial for vital cellular functions including gene transcription, DNA replication and recombination, repair, segregation, chromosomal stability, cell cycle progression, and epigenetic silencing. The ChIP assay has been used to study both histones and non-histone proteins, such as transcription factors, within the context of the cell. Transcription factors and other DNA binding proteins have a weaker affinity than histones, which generally are tightly associated within the chromatin complex. To avoid dissociation of non-histone proteins from the chromatin binding site, it is necessary to incorporate a cross-link step. QuikChIP(TM) Kit has been optimized for use with both histone and non-histone proteins. Using the QuikChIP(TM) Assay, cells are fixed with formaldehyde to cross-link nonhistone proteins to DNA. Alternatively, the cross-linking steps are not needed for histone proteins, which are tightly bound to DNA. The chromatin is released from the nuclei and sheared into 200-1000 bp fragments. The chromatin is then immunoprecipitated with specific antibodies. DNA sequences cross-linked to the protein of interest co-precipitate as part of the chromatin complex. The DNA/Chromatin/Antibody complex is then isolated using Protein G Agarose. After reverse cross-linking, the associated DNA is released from the complex and is ready for analysis.

    Additional QuikChIP Products

    Product Documents for QuikChIP Kit (ChIP)

    Certificate of Analysis

    To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

    Product Specific Notices for QuikChIP Kit (ChIP)

    This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

    Citations for QuikChIP Kit (ChIP)

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    Protocols

    View specific protocols for QuikChIP Kit (ChIP) (NBP2-29902):


    TRITON X-100

    Hazard Information
    Chemical Name: TRITON X-100
    Chemical Formula: (C2-H4-O)nC14-H22-O CAS Number: 9002-93-1
    EEC-No: n/a

    First Aid Measures
    Eye Contact: Can causes eye irritation.
    Skin Contact: Causes skin irritation and is toxic if absorbed through skin. Inhalation: May be
    harmful if inhaled. Causes respiratory tract irritation. Ingestion: Harmful if swallowed.
    Accidental Release Measures
    If inhaled, move person into fresh air. If not breathing give artificial respiration and consult a
    physician.
    In case of skin contact, wash off with soap and plenty of water.
    In case of eye contact, rinse thoroughly with plenty of water for at least 15 minutes.
    If swallowed do NOT induce vomiting. Rinse mouth with water and consult a physician.
    Handling and Storage
    Handling: Avoid contact with skin and eyes. Keep container tightly closed and store and handle in a
    cool, dry, well-ventilated place.

    Exposure Controls / Personal Protection Ventilation: Handle in a well-ventilated area Gloves:
    Handle with rubber or latex gloves
    Eye Protection: Safety glasses, goggles or face shield to protect from splash hazard

    Physical and Chemical Properties
    Form: Liquid
    Color: Colorless
    Odor: Odorless
    Melting Point: 6 degrees C (42.8 degrees F)
    Boiling Temperature: 270 degrees C (518 degrees F) Density: No data available
    Vapor Pressure: No data available Solubility in Water: Very soluble Flash Point: No data available
    Explosion limits: No data available
    Ignition Temperature: No data available

    Stability and Reactivity
    Stable under recommended storage conditions.

    Disposal/Spill Considerations
    Small Spill: Dilute with water and mop up, or absorb with an inert dry material and place in an
    appropriate waste disposal container. Finish cleaning by spreading water on the contaminated
    surface and dispose of according to local and regional authority requirements.
    Large Spill:Absorb with an inert material and put the spilled material in an appropriate waste
    disposal. Finish cleaning by spreading water on the contaminated surface and allow to evacuate
    through the sanitary system.


    Sodium Dodecyl Sulphate

    Hazard Information
    Chemical Name: Sodium Dodecyl Sulphate Chemical Formula: NaC12H25O4S
    CAS Number: 151-21-3
    EEC-No: 205-788-1

    First Aid Measures
    Eye Contact: Can causes eye irritation.
    Skin Contact: Causes skin irritation and is toxic if absorbed through skin. Inhalation: May be
    harmful if inhaled. Causes respiratory tract irritation. Ingestion: Harmful if swallowed.

    Accidental Release Measures
    If inhaled, move person into fresh air. If not breathing give artificial respiration and consult a
    physician.
    In case of skin contact, wash off with soap and plenty of water.
    In case of eye contact, rinse thoroughly with plenty of water for at least 15 minutes.
    If swallowed do NOT induce vomiting. Rinse mouth with water and consult a physician.

    Handling and Storage
    Handling: Avoid contact with skin and eyes. Avoid formation of dust and aerosols. Handle powder in
    a fume hood. Keep container tightly closed in a cool, dry, well-ventilated place.

    Exposure Controls / Personal Protection Ventilation: Handle powder in a fume hood Gloves: Handle
    with rubber or latex gloves
    Eye Protection: Safety glasses, goggles or face shield

    Physical and Chemical Properties
    Form: Solid Color: White Odor: Odorless
    Melting Point: No data available Boiling Temperature: No data available Density: No data available
    Vapor Pressure: No data available Solubility in Water: Very soluble Flash Point: No data available
    Explosion limits: No data available
    Ignition Temperature: No data available

    Stability and Reactivity
    Stable under recommended storage conditions.

    Disposal Considerations
    Contain spill collect with a vacuum cleaner or by wet-brushing and place in a container for
    disposal according to local regulations. Keep in suitable, closed containers for disposal.



    EDTA

    Hazard Information
    Chemical Name: Ethylenediaminetetraacetic Acid Tetrasodium Salt, Dihydrate Chemical Formula: C10H12N2Na4O8.2H2O
    CAS Number: 10378-23-1

    First Aid Measures
    Eye Contact: Can cause slight eye irritation. Skin Contact: Can cause slight skin irritation.
    Inhalation: Can cause slight respiratory tract irritation.
    Ingestion: Harmful if swallowed.

    Accidental Release Measures
    If inhaled, move person into fresh air. If not breathing give artificial respiration and consult a
    physician.
    In case of skin contact, wash off with soap and plenty of water.
    In case of eye contact, rinse thoroughly with plenty of water for at least 15 minutes.
    If swallowed do NOT induce vomiting. Rinse mouth with water and consult a physician.

    Handling and Storage
    Handling: Avoid contact with skin and eyes. Keep container tightly closed in a cool, dry, well-
    ventilated place.

    Exposure Controls / Personal Protection Ventilation: Handle in a well-ventilated area Gloves:
    Handle with rubber or latex gloves
    Eye Protection: Safety glasses, goggles or face shield

    Physical and Chemical Properties
    Form: Solid Color: White
    Odor: No data available
    Melting Point: No data available Boiling Temperature: No data available Density: No data available
    Vapor Pressure: No data available Solubility in Water: soluble
    Flash Point: > 93.3 degrees C (200 degrees F) Explosion limits: No data available Ignition Temperature: No data
    available

    Stability and Reactivity
    Stable under recommended storage conditions.
    Disposal Considerations
    Small Spill: Use appropriate tools to put the spilled solid in a convenient waste disposal container. Finish cleaning by spreading water on the contaminated surface and dispose of according to local and regional authority requirements.
    Large Spill: Use a shovel to put the material into a convenient waste disposal container. Finish cleaning by spreading water on the contaminated surface and allow to evacuate through the sanitary system.


    Glycine (Glycerine)

    Hazard Information
    Chemical Name: Glycine (Glycerine) Chemical Formula: C2H5NO2
    CAS Number: 56-40-6

    First Aid Measures
    Eye Contact: Can cause slight eye irritation. Skin Contact: Can cause slight skin irritation.
    Inhalation: Can cause slight respiratory tract irritation. Ingestion: Harmful if swallowed.

    Accidental Release Measures
    If inhaled, move person into fresh air. If not breathing give artificial respiration and consult a
    physician.
    In case of skin contact, wash off with soap and plenty of water.
    In case of eye contact, rinse thoroughly with plenty of water for at least 15 minutes.
    If swallowed do NOT induce vomiting. Rinse mouth with water and consult a physician.

    Handling and Storage
    Handling: Avoid contact with skin and eyes. Keep container tightly closed in a cool, dry, well-
    ventilated place.

    Exposure Controls / Personal Protection Ventilation: Handle in a well-ventilated area Gloves:
    Handle with rubber or latex gloves
    Eye Protection: Safety glasses, goggles or face shield to prevent splash hazard

    Physical and Chemical Properties
    Form: Liquid Color: Colorless Odor: Odorless
    Melting Point: Decomposition temperature: Starts to decompose at 233 degrees C (451.4 degrees F); Completely
    sintered at 280 deg. C.
    Boiling Temperature: No data available Density: No data available
    Vapor Pressure: No data available Solubility in Water: soluble
    Flash Point: >176.67 degrees C (350 degrees F)
    Explosion limits: No data available Ignition Temperature: No data available

    Stability and Reactivity
    Stable under recommended storage conditions.
    Disposal Considerations
    Small Spill: Use appropriate tools to put the spill in a convenient waste disposal container.
    Finish cleaning by spreading water on the contaminated surface and dispose of according to local
    and regional authority requirements.
    Large Spill: Use a shovel to put the material into a convenient waste disposal container. Finish cleaning by spreading water on the contaminated surface and allow to evacuate through the sanitary system.


    PMSF

    Hazard Information
    Chemical Name: Phenylmethylsulfonyl fluoride Chemical Formula: C7H7FO2S
    CAS Number: 329-98-6

    First Aid Measures
    Eye Contact: Can cause slight eye irritation. Skin Contact: Can cause slight skin irritation.
    Inhalation: Can cause slight respiratory tract irritation. Ingestion: Harmful if swallowed.

    Accidental Release Measures
    If inhaled, move person into fresh air. If not breathing give artificial respiration and consult a
    physician.
    In case of skin contact, wash off with soap and plenty of water.
    In case of eye contact, rinse thoroughly with plenty of water for at least 15 minutes.
    If swallowed do NOT induce vomiting. Rinse mouth with water and consult a physician.

    Handling and Storage
    Handling: Avoid contact with skin and eyes. Keep container tightly closed in a cool, dry, well-
    ventilated place.

    Exposure Controls / Personal Protection Ventilation: Handle in a well-ventilated area Gloves:
    Handle with rubber or latex gloves
    Eye Protection: Safety glasses, goggles or face shield

    Physical and Chemical Properties
    Form: Solid
    Color: No data available Odor: No data available Melting Point: 92 degrees C (197.6 degrees F)
    Boiling Temperature: No data available Density: No data available
    Vapor Pressure: No data available Solubility in Water: soluble
    Flash Point: No data available Explosion limits: No data available Ignition Temperature: No data
    available

    Stability and Reactivity
    Stable under recommended storage conditions.
    Disposal Considerations
    Small Spill: Use appropriate tools to put the spilled solid in a convenient waste disposal
    container.
    Large Spill: Corrosive solid. Stop leak if without risk. Do not get water inside container. Do not
    touch spilled material. Use water spray to reduce vapors. Prevent entry into sewers, basements or
    confined areas; dike if needed. Eliminate all ignition sources. Call for assistance on disposal.


    Protease Inhibitor Cocktail

    Hazard Information
    Chemical Name: 4-(2-Aminoethyl) benzenesulfonylfluoride hydrochloride Chemical Formula: C8H10FNO2S
    HCl
    CAS Number: 30827-99-7

    First Aid Measures
    Eye Contact: Can cause slight eye irritation. Skin Contact: Can cause slight skin irritation.
    Inhalation: Can cause slight respiratory tract irritation. Ingestion: Harmful if swallowed.

    Accidental Release Measures
    If inhaled, move person into fresh air. If not breathing give artificial respiration and consult a
    physician.
    In case of skin contact, wash off with soap and plenty of water.
    In case of eye contact, rinse thoroughly with plenty of water for at least 15 minutes.
    If swallowed do NOT induce vomiting. Rinse mouth with water and consult a physician.

    Handling and Storage
    Handling: Avoid contact with skin and eyes. Keep container tightly closed in a cool, dry, well-
    ventilated place.

    Exposure Controls / Personal Protection Ventilation: Handle in a well-ventilated area Gloves:
    Handle with rubber or latex gloves
    Eye Protection: Safety glasses, goggles or face shield

    Physical and Chemical Properties
    Form: Solid Color: beige
    Odor: No data available Melting Point: 183 degrees C (361 degrees F)
    Boiling Temperature: No data available Density: No data available
    Vapor Pressure: No data available Solubility in Water: soluble
    Flash Point: No data available Explosion limits: No data available Ignition Temperature: No data
    available

    Stability and Reactivity
    Stable under recommended storage conditions.
    Disposal Considerations
    Small Spill: Use appropriate tools to put the spilled solid in a convenient waste disposal container.
    Large Spill: Corrosive solid. Stop leak if without risk. Do not get water inside container. Do not
    touch spilled material. Use water spray to reduce vapors. Prevent entry into sewers, basements or
    confined areas; dike if needed. Eliminate all ignition sources. Call for assistance on disposal.


    Salmon sperm DNA

    Hazard Information
    Chemical Name: Salmon sperm DNA Chemical Formula: n/a
    CAS Number: 100403-24-5
    EEC-No: 309-566-6

    First Aid Measures
    Eye Contact: May cause eye irritation. Skin Contact: May cause skin irritation. Inhalation: May be
    harmful if inhaled. Ingestion: May be harmful if swallowed.

    Accidental Release Measures
    If inhaled, move person into fresh air. If not breathing give artificial respiration and consult a
    physician.
    In case of skin contact, wash off with soap and plenty of water.
    In case of eye contact, rinse thoroughly with plenty of water for at least 15 minutes.
    If swallowed do NOT induce vomiting. Rinse mouth with water and consult a physician.

    Handling and Storage
    Handling: Avoid contact with skin and eyes. Avoid formation of dust and aerosols. Handle powder in
    a well-ventilated area. Keep container tightly closed in a cool, dry, well-ventilated place.

    Exposure Controls / Personal Protection Ventilation: Handle powder in a well-ventilated area
    Gloves: Handle with rubber or latex gloves
    Eye Protection: Safety glasses, goggles or face shield

    Physical and Chemical Properties
    Form: Solid Color: White
    Odor: No data available
    Melting Point: No data available Boiling Temperature: No data available Density: No data available
    Vapor Pressure: No data available Solubility in Water: soluble
    Flash Point: No data available Explosion limits: No data available Ignition Temperature: No data
    available

    Stability and Reactivity
    Stable under recommended storage conditions.
    Disposal Considerations
    Absorb on sand or vermiculite and place in closed containers for disposal. Dispose contaminated
    material as waste according to all federal, state and local environmental regulations.. Ventilate
    area and wash spill site after material clean-up is complete.


    Proteinase K

    Hazard Information
    Chemical Name: Proteinase K, from Tritirachium album CAS Number: 39450-01-6

    First Aid Measures
    Eye Contact: Can cause slight eye irritation. Skin Contact: Can cause slight skin irritation.
    Inhalation: Can cause slight respiratory tract irritation.
    Ingestion: Harmful if swallowed.

    Accidental Release Measures
    If inhaled, move person into fresh air. If not breathing give artificial respiration and consult a
    physician.
    In case of skin contact, wash off with soap and plenty of water.
    In case of eye contact, rinse thoroughly with plenty of water for at least 15 minutes.
    If swallowed do NOT induce vomiting. Rinse mouth with water and consult a physician.

    Handling and Storage
    Handling: Avoid contact with skin and eyes. Keep container tightly closed in a cool, dry, well-ventilated place.

    Exposure Controls / Personal Protection Ventilation: Handle in a well-ventilated area Gloves:
    Handle with rubber or latex gloves
    Eye Protection: Safety glasses, goggles or face shield to prevent splash hazard

    Physical and Chemical Properties
    Form: Solid
    Color: No data available Odor: No data available
    Melting Point: No data available Boiling Temperature: No data available Density: No data available
    Vapor Pressure: No data available Solubility in Water: soluble
    Flash Point: No data available Explosion limits: No data available Ignition Temperature: No data
    available

    Stability and Reactivity
    Stable under recommended storage conditions.

    Disposal Considerations
    Small Spill: Use appropriate tools to put the spill in a convenient waste disposal container.
    Finish cleaning by spreading water on the contaminated surface and dispose of according to local and
    regional authority requirements.
    Large Spill: Use a shovel to put the material into a convenient waste disposal container. Finish
    cleaning by spreading water on the contaminated surface and allow to evacuate through the sanitary
    system.

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