Quinacrine Inhibitor

Novus Biologicals | Catalog # NBP2-29385

Novus Biologicals
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Product Specifications

Details of Functionality

Endosomal toll-like receptor inhibitor (antagonist); Inhibitor of endosomal acidification on which functional activity of endosomal TLRs (particularly TLR9 and TLR3) is dependent.

Application Notes

Assay>=90%

Scientific Data Images for Quinacrine Inhibitor

Quinacrine Inhibitor [NBP2-29385]
Other: Quinacrine Inhibitor [NBP2-29385]

Other: Quinacrine Inhibitor [NBP2-29385]

Quinacrine Inhibitor [NBP2-29385] - Evaluation of inhibitory activity of quinacrine. TLR9/NF-kB/SEAPorter™ HEK 293 and TLR2/NF-kB/SEAPorter™ HEK 293 cells were plated in 96-well plates at 5x10^4 cells/well for 16 h. Cells were preincubated with different concentrations of quinacrine (0.05, 0.1, 0.5, 1, 5 and 10 uM) for 30 min. Cell were then stimulated with 10 ug/ml CpG [A] or 20 ng/ml MALP-2 [B] for 24 h. Secreted alkaline phosphatase (SEAP) was analyzed using SEAPorter™ Assay Kit
Other: Quinacrine Inhibitor [NBP2-29385]

Other: Quinacrine Inhibitor [NBP2-29385]

Quinacrine Inhibitor [NBP2-29385] - TLR9/NF-kB/SEAPorter™ HEK 293 cells were plated in 96-well plates at 5x10^4 cells/well for 16 h. Cells were preincubated with various concentrations of quinacrine for 30 min, followed by stimulation with 10 ug/ml CpG for 24 h. SEAP was analyzed using SEAPorter™ Assay Kit. Dose-responsive percent inhibition of each sample well was calculated to yield the quinacrine IC50 value.

Formulation, Preparation, and Storage

Formulation

Form : Yellow Solid (Powder)
Solubility:Deionized water

Concentration

Concentration is not relevant for this product. Please see the protocols for proper use of this product.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at 4C. Do not freeze.

Background: Quinacrine

Quinacrine is a weak base which can partition into acidic vesicles such as endosomes and lysosomes, resulting in inhibition of endosomal acidification and lysosomal enzyme activity. Because acidic pH of endosomes is a prerequisite of endosomal TLR activation, quinacrine can serve as an antagonist for endosomal TLRs. Quinacrine and its analog chloroquine are also known to act as therapeutic agents for autoimmune diseases such as rheumatoid arthritis and systemic lupus erythematosus, of which therapeutic activity is due to suppression of TLR9 activity as shown by researchers.

Alternate Names

1-2-bromophenyl-5-3-methylphenyl triazole-4-carboxylic acid, 6-Chloro-9-4-diethylamino-1-methylbutylamino-2-methoxyacridine dihydrochloride, Atebrin dihydrochloride, C23H30ClN3O-2HCl, Mepacrine dihydrochloride, Quinacrine dihydrochloride

Additional Quinacrine Products

Product Documents for Quinacrine Inhibitor

Certificate of Analysis

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Product Specific Notices for Quinacrine Inhibitor

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Inhibitors are guaranteed for 1 year from date of receipt.

Citations for Quinacrine Inhibitor

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Protocols

View specific protocols for Quinacrine Inhibitor (NBP2-29385):

Product Handling Protocol (NBP2-29385):
1. To make 100 mM stock solution, dissolve 200 mg quinacrine in 4.2 ml water by gentle vortex.
2. Filter sterilize through a 0.22 um filter.
3. Store at 4oC (Note: Quinacrine solution is light sensitive.).
4. For TLR signaling inhibition study, perform a pilot inhibitory test with the different concentrations of quinacrine ranging from 1 to 10 mM to optimize your experiments.