RAB3IP Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-92309

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all RAB3IP Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: AEISSISFHVTDPAPCSTSGVTAGLTKLTTRKDNYNAEREFLQGATITEACDGSDDIFGLSTDSLSRLRSPSVLEVREKGYERLKEELAKAQ

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (80%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

53 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for RAB3IP Antibody - BSA Free

Western Blot: RAB3IP Antibody [NBP1-92309]

Western Blot: RAB3IP Antibody [NBP1-92309]

Western Blot: RAB3IP Antibody [NBP1-92309] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue
Immunocytochemistry/ Immunofluorescence: RAB3IP Antibody [NBP1-92309]

Immunocytochemistry/ Immunofluorescence: RAB3IP Antibody [NBP1-92309]

Immunocytochemistry/Immunofluorescence: RAB3IP Antibody [NBP1-92309] - Staining of human cell line A-431 shows localization to intermediate filaments. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: RAB3IP Antibody [NBP1-92309]

Immunohistochemistry-Paraffin: RAB3IP Antibody [NBP1-92309]

Immunohistochemistry-Paraffin: RAB3IP Antibody [NBP1-92309] - Staining of human skeletal muscle shows no positivity in myocytes as expected.
Immunohistochemistry-Paraffin: RAB3IP Antibody [NBP1-92309]

Immunohistochemistry-Paraffin: RAB3IP Antibody [NBP1-92309]

Immunohistochemistry-Paraffin: RAB3IP Antibody [NBP1-92309] - Staining of human pancreas shows strong cytoplasmic positivity in pancreatic ductal cells.
Immunohistochemistry-Paraffin: RAB3IP Antibody [NBP1-92309]

Immunohistochemistry-Paraffin: RAB3IP Antibody [NBP1-92309]

Immunohistochemistry-Paraffin: RAB3IP Antibody [NBP1-92309] - Staining of human kidney shows moderate membranous and cytoplasmic positivity in cells in tubules.
Immunohistochemistry-Paraffin: RAB3IP Antibody [NBP1-92309]

Immunohistochemistry-Paraffin: RAB3IP Antibody [NBP1-92309]

Immunohistochemistry-Paraffin: RAB3IP Antibody [NBP1-92309] - Staining of human pancreas shows strong cytoplasmic and membranous positivity in exocrine glandular cells.
Immunohistochemistry-Paraffin: RAB3IP Antibody [NBP1-92309]

Immunohistochemistry-Paraffin: RAB3IP Antibody [NBP1-92309]

Immunohistochemistry-Paraffin: RAB3IP Antibody [NBP1-92309] - Staining of human placenta shows strong membranous and cytoplasmic positivity in trophoblastic cells.

Applications for RAB3IP Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500-1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval method is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RAB3IP

RAB3IP, also known as Rab-3A-interacting protein, is a 53 kDa 476 amino acid protein with 8 isoforms produced by alternative splicing. The primary function of RAB3IP is to change GDP proteins into GTP proteins. Current research on RAB3IP is being conducted in relation to vitelliform, macular dystrophy, pilocytic astrocytoma, anaplastic astrocytoma and astrocytoma. RAB3IP is linked to the vesicle docking, metaphase, exocytosis, mitosis, transport, synaptic transmission and cytokinesis pathways where it interacts with RAB3IL1, SSX2B, SSX2, RAB8A and RAB3A.

Alternate Names

FLJ14660, FLJ22548, MGC71495, RAB3A interacting protein (rabin3), rab-3A-interacting protein, Rab3A-interacting protein, RABIN3, Rabin-3, SSX2 interacting protein, SSX2-interacting protein

Entrez Gene IDs

117177 (Human)

Gene Symbol

RAB3IP

UniProt

Q96QF0 (Human)

Additional RAB3IP Products

Product Documents for RAB3IP Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for RAB3IP Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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