Rab4 Antibody - Azide and BSA Free

Novus Biologicals | Catalog # H00005867-B01P

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Mouse IgG

Format

Azide and BSA Free
View all Rab4 Primary Antibodies »

Product Specifications

Immunogen

RAB4A (NP_004569.2, 1 a.a. - 218 a.a.) full-length human protein. MSQTAMSETYDFLFKFLVIGNAGTGKSCLLHQFIEKKFKDDSNHTIGVEFGSKIINVGGKYVKLQIWDTAGQERFRSVTRSYYRGAAGALLVYDITSRETYNALTNWLTDARMLASQNIVIILCGNKKDLDADREVTFLEASRFAQENELMFLETSALTGENVEEAFVQCARKILNKIESGELDPERMGSGIQYGDAALRQLRSPRRAQAPNAQECGC

Marker

Early and Recycling Endosomes Marker

Specificity

RAB4A - RAB4A, member RAS oncogene family,

Clonality

Polyclonal

Host

Mouse

Isotype

IgG

Description

Quality control test: Antibody reactive against mammalian transfected lysate.

Scientific Data Images for Rab4 Antibody - Azide and BSA Free

Western Blot: Rab4 Antibody [H00005867-B01P]

Western Blot: Rab4 Antibody [H00005867-B01P]

Western Blot: Rab4 Antibody [H00005867-B01P] - Analysis of RAB4A expression in rat brain.
Western Blot: Rab4 Antibody [H00005867-B01P]

Western Blot: Rab4 Antibody [H00005867-B01P]

Western Blot: Rab4 Antibody [H00005867-B01P] - Analysis of RAB4A expression in transfected 293T cell line by RAB4A polyclonal antibody. Lane 1: RAB4A transfected lysate(23.98 KDa). Lane 2: Non-transfected lysate.

Applications for Rab4 Antibody - Azide and BSA Free

Application
Recommended Usage

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB and also on transfected lysate in WB. GST tag alone is used as a negative control.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4)

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Rab4

Rab4 is a small GTPase that regulates recycling of proteins, such as GPCRs, from the early endosomes to the cell surface. Overexpression of Rab4 causes a redistribution of receptors on plasma membrane versus endocytic compartments. Rab4 is also involved in the translocation of glucose transporter (Glu4) in adipocytes in response to insulin. Association of Rab4 with transferrin receptor containing early endosomes is mediated through the geranylgeranyl groups at its carboxyl terminus. Rab4 can be used as a marker for early endosomes (see Daro et al. 1996 and Zuk et al. 1999 for more information). Membrane association is also cell cycle dependent, as phosphorylation at its C-terminal cdc2 kinase consensus sequence in mitotic cells leads to dissociation of Rab4 into the cytosol.

Alternate Names

Oncogene RAB4, RAB4, member RAS oncogene family, RAB4A, member RAS oncogene family, RAB4HRES-1/RAB4, ras-related protein Rab-4A

Entrez Gene IDs

5867 (Human)

Gene Symbol

RAB4A

UniProt

NP_004569.2 (Human)

Additional Rab4 Products

Product Documents for Rab4 Antibody - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Rab4 Antibody - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FAQs

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