Rad17 [p Ser645] Antibody - BSA Free
Novus Biologicals | Catalog # NB100-273
![Western Blot: Rad17 [p Ser645] Antibody [NB100-273]](https://resources.rndsystems.com/images/products/Rad17-[p-Ser645]-Antibody-Western-Blot-NB100-273-img0045.jpg "Western Blot: Rad17 [p Ser645] Antibody [NB100-273]")
Key Product Details
Validated by
Biological Validation
Species Reactivity
Human, Mouse
Applications
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
A synthetic peptide, which represented a portion of human Rad17 (LocusLink ID 5884) around serine 645.
Modification
p Ser645
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Rad17 [p Ser645] Antibody - BSA Free
Western Blot: Rad17 [p Ser645] Antibody [NB100-273]
Western Blot: Rad17 [p Ser645] Antibody [NB100-273] - Samples: Whole cell lysate (50 ug) from HeLa and 293T cells treated with 100 uM EPE for 2 hours (+) or mock treated (-) cells. Antibodies : Affinity purified rabbit anti-Rad17, Phospho (S645) antibody. Detection: Chemiluminescence with exposure times of 30 seconds (upper and lower panels).![Immunohistochemistry: Rad17 [p Ser645] Antibody [NB100-273]](https://resources.rndsystems.com/images/products/Rad17-[p-Ser645]-Antibody-Immunohistochemistry-NB100-273-img0049.jpg "Immunohistochemistry: Rad17 [p Ser645] Antibody [NB100-273]")
Immunohistochemistry: Rad17 [p Ser645] Antibody [NB100-273]
Immunohistochemistry: Rad17 [p Ser645] Antibody [NB100-273] - Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-Phospho Rad17 (S645) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB![Western Blot: Rad17 [p Ser645] Antibody [NB100-273]](https://resources.rndsystems.com/images/products/Rad17-[p-Ser645]-Antibody-Western-Blot-NB100-273-img0002.jpg "Western Blot: Rad17 [p Ser645] Antibody [NB100-273]")
![Immunohistochemistry: Rad17 [p Ser645] Antibody [NB100-273]](https://resources.rndsystems.com/images/products/Rad17-[p-Ser645]-Antibody-Immunohistochemistry-NB100-273-img0048.jpg "Immunohistochemistry: Rad17 [p Ser645] Antibody [NB100-273]")
Immunohistochemistry: Rad17 [p Ser645] Antibody [NB100-273]
Immunohistochemistry: Rad17 [p Ser645] Antibody [NB100-273] - Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-Phospho Rad17 (S645) used at a dilution of 1:1,000 (1ug/ml). Detection: DABApplications for Rad17 [p Ser645] Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry
1:500 - 1:2000
Western Blot
1:500-1:5000
Application Notes
IHC: Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: Rad17
Alternate Names
CCYC, cell cycle checkpoint protein (RAD17), cell cycle checkpoint protein RAD17, FLJ41520, hRad17, R24L, RAD1 (S. pombe) homolog, RAD1 homolog, RAD17 homolog (S. pombe), Rad17-like protein, RAD17Sp, Rad24, RF-C activator 1 homolog, RF-C/activator 1 homolog
Entrez Gene IDs
5884 (Human)
Gene Symbol
RAD17
Additional Rad17 Products
Product Documents for Rad17 [p Ser645] Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Rad17 [p Ser645] Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for Rad17 [p Ser645] Antibody - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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