RAD18 Antibody (79B1048.1) - BSA Free

Novus Biologicals | Catalog # NB100-56523

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 79B1048.1

Format

BSA Free
View all RAD18 Primary Antibodies »

Product Specifications

Immunogen

The antibody was raised against a synthetic peptide corresponding to the amino acids 402-414 of human Rad18.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for RAD18 Antibody (79B1048.1) - BSA Free

Western Blot: RAD18 Antibody (79B1048.1) [NB100-56523]

Western Blot: RAD18 Antibody (79B1048.1) [NB100-56523]

Western Blot: RAD18 Antibody (79B1048.1) [NB100-56523] - The anti-Rad18 mAb was diluted at 2 ug/ml and tested against 20 ug of HL-60 cell lysate.

Applications for RAD18 Antibody (79B1048.1) - BSA Free

Application
Recommended Usage

Western Blot

2-4 ug/ml
Application Notes
In HL60, a 54 kDa band should be detected.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS containing 0.2% gelatin

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RAD18

Human Rad18 (hRad18) is homologue of the S. pombe Rad18 (3,4). In yeast, Rad6 and Rad18 play a crucial role in post-replication repair functions in gap-filling of a daughter strand on replication of damaged DNA (2). Furthermore, Rad6-Rad18 interaction is thought to be critical at an early step during lesion bypass in yeast. In vivo, Rad18 protein forms a tight complex with Rad6 through a conserved ring-finger motif (3,4). It has been suggested that Rad18-Rad6 complex binds to the damaged sites by the single-stranded DNA binding property of Rad 18. This is followed by degradation of the stalled components of DNA replication by ubiquitin-conjugating enzyme activity of Rad6 (1). The human Rad18 maps on chromosome 3p24-25, the region which is often deleted in lung, breast, ovary, and testis cancers. The human RAD18 gene codes for a protein of 495 amino acids with a predicted molecular weight of 56 kDa. The human Rad18 is ubiquitously expressed in various human tissues.

Alternate Names

EC 6.3.2.-, hHR18, hRAD18, postreplication repair protein hRAD18p, Postreplication repair protein RAD18, RAD18 homolog (S. cerevisiae), RAD18, S. cerevisiae, homolog, RING finger protein 73, RNF73E3 ubiquitin-protein ligase RAD18

Entrez Gene IDs

56852 (Human)

Gene Symbol

RAD18

Additional RAD18 Products

Product Documents for RAD18 Antibody (79B1048.1) - BSA Free

Certificate of Analysis

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Product Specific Notices for RAD18 Antibody (79B1048.1) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for RAD18 Antibody (79B1048.1) - BSA Free

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