Rad21 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-83260

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (91%), Rat (92%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Chromatin Immunoprecipitation-exo-Seq

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Rad21 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: KLMMWKETGGVEKLFSLPAQPLWNNRLLKLFTRCLTPLVPEDLRKRRKGGEADNLDEFLKEFENPEVPREDQQQQHQQRDVIDEPIIEEPSRLQESVMEASRTNIDESAMPPPPPQGVKRKAGQIDPEPVMPPQQVEQMEIPPVEL

Localization

Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Rad21 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Rad21 Antibody [NBP1-83260]

Immunocytochemistry/ Immunofluorescence: Rad21 Antibody [NBP1-83260]

Immunocytochemistry/Immunofluorescence: Rad21 Antibody [NBP1-83260] - Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.
Immunohistochemistry-Paraffin: Rad21 Antibody [NBP1-83260]

Immunohistochemistry-Paraffin: Rad21 Antibody [NBP1-83260]

Immunohistochemistry-Paraffin: Rad21 Antibody [NBP1-83260] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.
Immunohistochemistry-Paraffin: Rad21 Antibody [NBP1-83260]

Immunohistochemistry-Paraffin: Rad21 Antibody [NBP1-83260]

Immunohistochemistry-Paraffin: Rad21 Antibody [NBP1-83260] - Staining of human cerebellum shows strong nuclear positivity in cells in granular layer.
Immunohistochemistry-Paraffin: Rad21 Antibody [NBP1-83260]

Immunohistochemistry-Paraffin: Rad21 Antibody [NBP1-83260]

Immunohistochemistry-Paraffin: Rad21 Antibody [NBP1-83260] - Staining of human placenta shows strong nuclear positivity in trophoblastic cells.
Immunohistochemistry-Paraffin: Rad21 Antibody [NBP1-83260]

Immunohistochemistry-Paraffin: Rad21 Antibody [NBP1-83260]

Immunohistochemistry-Paraffin: Rad21 Antibody [NBP1-83260] - Staining of human tonsil shows strong nuclear positivity in non-germinal center cells.
Rad21 Antibody - BSA Free Western Blot: Rad21 Antibody - BSA Free [NBP1-83260]

Western Blot: Rad21 Antibody - BSA Free [NBP1-83260]

Analysis in control (vector only transfected HEK293T lysate) and RAD21 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells).
Rad21 Antibody - BSA Free Chromatin Immunoprecipitation-exo-Seq: Rad21 Antibody - BSA Free [NBP1-83260]

Chromatin Immunoprecipitation-exo-Seq: Rad21 Antibody - BSA Free [NBP1-83260]

ChIP-Exo-Seq composite graph for Anti-RAD21 (NBP1-83260) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

Applications for Rad21 Antibody - BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation-exo-Seq

1-10ug per reaction

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Rad21

The cohesin complex plays an important role in tethering sister chromatids during the prophase through anaphase stages of mitosis, making certain that genomic information is replicated accurately. As the cellular division process continues, separase destroys the cohesin complex by means of cleavaging its Scc1 subunit (Mcd1/Scc1/hRad21), allowing the chromatids to separate and divide with the cell. During apoptosis the C-terminal portion of hRad21 is cleaved and translocated to the cytoplasm. This cleavage and translocation may act as a nuclear signal that promotes and accelerates subsequent events of apoptosis (2).

Alternate Names

double-strand-break repair protein rad21 homolog, hHR21FLJ25655, HR21, KIAA0078RAD21 (S. pombe) homolog, MCD1, Nuclear matrix protein 1, NXP-1, NXP1HRAD21, protein involved in DNA double-strand break repair, RAD21 homolog (S. pombe), SCC1 homolog, SCC1FLJ40596

Entrez Gene IDs

5885 (Human)

Gene Symbol

RAD21

UniProt

O60216 (Human)

Additional Rad21 Products

Product Documents for Rad21 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Rad21 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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