Rad50 Antibody (5M2K10)

Novus Biologicals | Catalog # NBP3-16286

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 5M2K10 expressed in HEK293
View all Rad50 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1200-1300 of human Rad50 (Q92878). RCSAGQKVLASLIIRLALAETFCLNCGIIALDEPTTNLDRENIESLAHALVEIIKSRSQQRNFQLLVITHDEDFVELLGRSEYVEKFYRIKKNIDQCSEIV

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Rad50 Antibody (5M2K10)

Western Blot: Rad50 Antibody (5M2K10) [NBP3-16286]

Western Blot: Rad50 Antibody (5M2K10) [NBP3-16286]

Western Blot: Rad50 Antibody (5M2K10) [NBP3-16286] - Western blot analysis of extracts of various cell lines, using Rad50 Rabbit mAb (NBP3-16286) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Immunoprecipitation: Rad50 Antibody (5M2K10) [NBP3-16286]

Immunoprecipitation: Rad50 Antibody (5M2K10) [NBP3-16286]

Immunoprecipitation: Rad50 Antibody (5M2K10) [NBP3-16286] - Immunoprecipitation analysis of 300ug extracts of K-562 cells using 3ug Rad50 antibody (NBP3-16286). Western blot was performed from the immunoprecipitate using Rad50 antibody (NBP3-16286) at a dilition of 1:1000.

Applications for Rad50 Antibody (5M2K10)

Application
Recommended Usage

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Rad50

Rad50 is a 153kDa protein that associates with Mre11 and p95 (Nibrin) to form a multiprotein complex involved in the double strand break repair process. Upon DNA damage, the complex translocates to the nucleus of damaged cells, binds DNA, and displays numerous enzymatic activities that are required for nonhomologous joining of DNA ends. Overall, Rad50 is important for DNA double-strand break repair, cell cycle checkpoint activation, telomere maintenance, and meiotic recombination.

Alternate Names

DNA repair protein RAD50, EC 3.6, EC 3.6.1.15, EC 3.6.3, EC 3.6.3.27, hRAD50, NBSLD, RAD50 (S. cerevisiae) homolog, RAD50 homolog (S. cerevisiae), RAD502, RAD50-2

Gene Symbol

RAD50

Additional Rad50 Products

Product Documents for Rad50 Antibody (5M2K10)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Rad50 Antibody (5M2K10)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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