RAD52 Antibody (5H9) - Azide and BSA Free

Novus Biologicals | Catalog # NBP1-41208

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 5H9

Format

Azide and BSA Free
View all RAD52 Primary Antibodies »

Product Specifications

Immunogen

Human RAD52 recombinant protein expressed in E. coli.

Specificity

This antibody will recognize human Rad52.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for RAD52 Antibody (5H9) - Azide and BSA Free

RAD52 Antibody (5H9)

Western Blot: RAD52 Antibody (5H9) [NBP1-41208] -

Western Blot: RAD52 Antibody (5H9) [NBP1-41208] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with RAD52 antibody [5H9] (NBP1-41208) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Applications for RAD52 Antibody (5H9) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS, 20% Glycerol

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RAD52

DNA double-strand breaks can be generated by exposure to ionizing radiation or through endogenous cellular reactions. Double-strand breaks are generally thought to be repaired via one of two distinct mechanisms: homologous recombination (HR) or non-homologous end joining (NHEJ). Homologous recombination requires the activity of the members of the RAD52 epistasis group, which includeRAD50, RAD51, RAD52, RAD54, RDH54/TID1, RAD55, RAD57, RAD59, MRE11, and XRS2. RAD52 can catalyze strand annealing reactions, and can form oligomers that attach to ends of single-stranded DNA.

Alternate Names

DNA repair protein RAD52 homolog, RAD52 (S. cerevisiae) homolog, RAD52 homolog (S. cerevisiae), recombination protein RAD52, rhabdomyosarcoma antigen MU-RMS-40.23

Entrez Gene IDs

5893 (Human)

Gene Symbol

RAD52

Additional RAD52 Products

Product Documents for RAD52 Antibody (5H9) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for RAD52 Antibody (5H9) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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