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RADA | Fluorescent Probes for Imaging Bacteria
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Description: Orange-red TAMRA-based fluorescent D-amino acid for labeling peptidoglycans in live bacteria
Alternative Names: TADA

Purity: ≥98%

Product Details
Citations (2)

Biological Activity

RADA is an orange-red TAMRA-based fluorescent D-amino acid. Suitable for labeling peptidoglycans in live bacteria. Incorporated into bacterial cell walls during synthesis. Suitable for prolonged exposure due to red-shift photophysical properties. Suitable for dSTORM and reSTORM. Displays good water solubility, photostability and thermostability. Excitation/emission λ ~554/580 nm.

This product is supplied as a TFA salt, the stoichiometry of which is batch dependant. Please refer to the Certificate of Analysis to obtain the batch specific Net Product Content.

Scientific Data

Fluorescence Imaging Application of RADA View Larger

Application of RADA. Using FDAAs RADA and HADA (Catalog # 6647) to study peptidoglycan enzyme and substrate distribution, and the role of polar cell growth and peptidoglycan segregation over time. Image shows peptidoglycan segregation over time. Left to right - 2 mins HADA pulse (cyan), 45 min outgrowth, followed by 2 min RADA chase (magenta) in WT Msm cells. New cell wall (magenta), oldest cell wall (cyan). Scale = 5 μm.

For more information on this work and to request full protocols please read García-Heredia et al (2018) Peptidoglycan precursor synthesis along the sidewall of pole-growing mycobacteria. Elife 10 e37243 PMID: 30198841

Optical Data for RADA

RADA Dye Spectra
Emission Color Orange
λabs 554 nm
λem 580 nm
Extinction Coefficient (ε) 95000 M-1cm-1
Quantum Yield (φ) 0.69

Technical Data

Soluble to 100 mM in DMSO
Store at -20°C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.

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Citations for RADA

The citations listed below are publications that use Tocris products. Selected citations for RADA include:

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