HADA

Tocris Bioscience | Catalog # 6647

Blue fluorescent D-amino acid for labeling peptidoglycans in live bacteria
Tocris Bioscience

Key Product Details

Description

Blue fluorescent D-amino acid for labeling peptidoglycans in live bacteria

Wavelength

Excitation = 405 nm, Emission = 450 nm
View all Fluorescent Probes for Imaging Bacteria »

Product Description

HADA is a fluorescent D-amino acid. Suitable for labeling peptidoglycans in live bacteria. Results in strong peripheral and septal labeling of diverse bacterial cell populations without affecting growth rates. Displays mitochondrial outer membrane permeability. Excitation/emission λ ~405/450 nm.

Scientific Data Images for HADA

Investigating mycobacteria cell wall formation using FDAA HADA.

Investigating mycobacteria cell wall formation using FDAA HADA.

Investigating mycobacteria cell wall formation using fluorescent D-amino acid HADA. Image showing FDAA HADA incorporation in log-phase growth of WT, delta L,D-transpeptidases (LDT) and delta L,D-transpeptidases (LDT) comp cells. Scale = 5 μm. Baranowski et al show FDAAs are incorporated asymmetrically by L,D-transpeptidases. For more information on this work and to request full protocols read Baranowski et al (2018) Maturing Mycobacterium smegmatis peptidoglycan requires non-canonical crosslinks to maintain shape. Elife 16 e37516 PMID: 30324906
Using FDAAs HADA and RADA (Cat. No 6649) to study peptidoglycan enzyme and substrate distribution, and the role of polar cell growth and peptidoglycan segregation over time.

Using FDAAs HADA and RADA to study peptidoglycan enzyme and substrate distribution, and the role of polar cell growth and peptidoglycan segregation over time.

Image shows peptidoglycan segregation over time. Left to right - 2 mins HADA pulse (cyan), 45 min outgrowth, followed by 2 min RADA (Catalog # 6649) chase (magenta) in WT Msm cells. New cell wall (magenta), oldest cell wall (cyan). Scale = 5 μm. For more information on this work and to request full protocols please read García-Heredia et al (2018) Peptidoglycan precursor synthesis along the sidewall of pole-growing mycobacteria. Elife 10 e37243 PMID: 30198841

Optical Data

HADA Dye Spectra
Emission Color Blue
λabs 405 nm
λem 450 nm
Extinction Coefficient (ε) 36,700 M-1cm-1
Application Fluorescence Microscopy

Spectra Viewer

Plan Your Experiments

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Product Specifications for HADA

Molecular Weight

328.71

Formula

C13H12N2O6.HCl

Storage

Store at -20°C

Purity

≥98% (HPLC)

Chemical Name

3-[[(7-Hydroxy-2-oxo-2H-1-benzopyran-3-yl)carbonyl]amino]-D-alanine hydrocholoride

CAS Number

2253733-10-5

PubChem ID

137919867

InChI Key

PLVOEKQNBIXHDI-SBSPUUFOSA-N

SMILES

N[C@@H](C(O)=O)CNC(C1=CC(C=CC(O)=C2)=C2OC1=O)=O.Cl

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 32.87 100

Preparing Stock Solutions for HADA

The following data is based on the product molecular weight 328.71.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 3.04 mL 15.21 mL 30.42 mL
5 mM 0.61 mL 3.04 mL 6.08 mL
10 mM 0.30 mL 1.52 mL 3.04 mL
50 mM 0.06 mL 0.30 mL 0.61 mL

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Background References

References are publications that support the biological activity of the product. See our Citations tab to view 56 publications citing the usage of this product.

Product Documents for HADA

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for HADA

For research use only

Customer Reviews for HADA (1)

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  • Great for labelling bacterial cell walls.
    Name: Patrick Moynihan
    Species: Mycobacterium species
    Assay Type: In Vitro
    Verified Customer | Posted 05/11/2020
    We use this product to label the peptidoglycan of growing mycobacterial species. We generally follow published protocols for this, of which there are several. This will label regions of active peptidoglycan synthesis in the cell.
    HADA 6647

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