HADA

Catalog # Availability Size / Price Qty
6647/5
HADA | CAS No. 2253733-10-5 | Fluorescent Probes for Imaging Bacteria
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Description: Blue fluorescent D-amino acid for labeling peptidoglycans in live bacteria

Chemical Name: 3-[[(7-Hydroxy-2-oxo-2H-1-benzopyran-3-yl)carbonyl]amino]-D-alanine hydrocholoride

Purity: ≥98%

Product Details
Citations (2)
Reviews (1)

Biological Activity

HADA is a fluorescent D-amino acid. Suitable for labeling peptidoglycans in live bacteria. Results in strong peripheral and septal labeling of diverse bacterial cell populations without affecting growth rates. Displays mitochondrial outer membrane permeability. Excitation/emission λ ~405/450 nm.

Applications

Application of HADA

Figure 1: Investigating mycobacteria cell wall formation using FDAA HADA. Image showing FDAA HADA incorporation in log-phase growth of WT, Δ L,D-transpeptidases (LDT) and Δ L,D-transpeptidases (LDT)comp cells. Scale = 5 μm.

Baranowski et al show FDAAs are incorporated asymmetrically by L,D-transpeptidases. For more information on this work and to request full protocols read Baranowski et al (2018) Maturing Mycobacterium smegmatis peptidoglycan requires non-canonical crosslinks to maintain shape Elife 16 e37516 PMID: 30324906

Application of RADA

Figure 2: Using FDAAs HADA and RADA (Cat. No 6649) to study peptidoglycan enzyme and substrate distribution, and the role of polar cell growth and peptidoglycan segregation over time. Image shows peptidoglycan segregation over time. Left to right - 2 mins HADA pulse (cyan), 45 min outgrowth, followed by 2 min RADA chase (magenta) in WT Msm cells. New cell wall (magenta), oldest cell wall (cyan). Scale = 5 μm.

For more information on this work and to request full protocols please read García-Heredia et al (2018) Peptidoglycan precursor synthesis along the sidewall of pole-growing mycobacteria. Elife 10 e37243 PMID: 30198841

Optical Data for HADA

HADA Dye Spectra
Emission Color Blue
λabs 405 nm
λem 450 nm
Extinction Coefficient (ε) 36,700 M-1cm-1

Technical Data

M.Wt:
328.71
Formula:
C13H12N2O6.HCl
Solubility:
Soluble to 100 mM in DMSO
Purity:
≥98%
Storage:
Store at -20°C
CAS No:
2253733-10-5

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.

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Citations for HADA

The citations listed below are publications that use Tocris products. Selected citations for HADA include:

2 Citations: Showing 1 - 2

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Reviews for HADA

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Great for labelling bacterial cell walls.
By Patrick Moynihan on 05/11/2020
Application: Species: Other

We use this product to label the peptidoglycan of growing mycobacterial species. We generally follow published protocols for this, of which there are several. This will label regions of active peptidoglycan synthesis in the cell.


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