Rainbow Calibration Particle Set (6 peaks)

Novus Biologicals | Catalog # NBP3-00505

Novus Biologicals
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Key Product Details

Applications

Flow Cytometry, Microscopy
View all Rainbow Calibration Particle Set (6 peaks) Fluorescent Probes and Dyes »

Product Specifications

Description

Rainbow Calibration Particles are a mixture of fluorophore incorporated particles with 6 different fluorescent intensities that are used for calibrating flow cytometers:
  • Excite particles at any wavelength from 365 to 650 nm.
  • Calibrate multiple channels with the same set of particles.
  • Enable non-spectral matching to common fluorophores such as FITC, PE, PE-Cy5.
  • Deliver durable performance since fluorophores are encased within particles, not on the surface.
  • Remain stable for several years.
  • Packaged in a dropper bottle for easy dispensing and storage.
Concentration: 1 x 10^7 particles / mL

Particle size: 3.2 (+/- 0.1) micron

Particle material: polystyrene

Uses: Long term performance tracking or routine calibration of flow cytometers. Routine alignment and calibration in fluorescence and confocal fluorescence microscopy.

Application Notes

Shake vigorously or vortex briefly before use. Inclusion of a small amount of detergent in the diluent will help increase the number of singles. Diluted particles can be stored at 4C for future use.

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Scientific Data Images for Rainbow Calibration Particle Set (6 peaks)

Rainbow Calibration Particle Set (6 peaks) [NBP3-00505] - Front scatter x side scatter population dot plot, as well as a PE Log x PE-TR Log scatter dot plot, with 6 gated populations. Based on those gates we see histograms with 6 peaks for FITC, PE, PE-Cy5, and PE-TR channels.

Formulation, Preparation, and Storage

NBP3-00505
Formulation Deionized water, 0.01% NP-40
Preservative 0.02% Sodium Azide
Concentration Please see the protocols for proper use of this product. If no protocol is available, contact technical services for assistance.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C in the dark. Do not freeze.

Background: Rainbow Calibration Particle Set (6 peaks)

Rainbow Calibration Particles are used to ascertain the relative voltage range for each flow cytometer detector. This mixture of fluorescent particles serves as a reliable and reproducible means of evaluating the sensitivity and resolution of flow cytometers.

The relative number of fluorophores can been determined for every emission peak of the rainbow calibration particles in FL1 (FITC, MEFL), FL2 (RPE, MEPE), FL3 (RPE-Cy5, MEPCY) and FL4 (APC, MEAP) channels in order to plot the calibration graph. The calibration graph is used to check the linearity (dynamic range) of the photomultiplier tube (PMT) in each channel. Additionally, the relative number of fluorophores can be cross calibrated with cells or particles stained with a known number of spectral matching fluorophores such as FITC, PE, RPE-Cy5 to estimate the number of fluorophores on stained cells.

Alternate Names

Calibration Beads, Flow Cytometry Calibration, Rainbow Calibration Beads

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Product Specific Notices for Rainbow Calibration Particle Set (6 peaks)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.

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