Q: Were the cells lysed immediately after collection, or was the cell pellet frozen and lysed at a later date?

A: The lysates are prepared from frozen cell pellets.

Raji Whole Cell Lysate

Novus Biologicals | Catalog # NB820-59466

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Product Documents

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Product Summary
Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species

Human

Applications

Western Blot

Product Summary for Raji Whole Cell Lysate

We recommend adding 1 x sample buffer with 5% BME (or other reducing agent) prior to use.

Product Specifications

Application Notes

Western Blot, Immunoprecipitation, Electrophoresis, Enzymatic activity analysis, Protein-protein interaction, Tissue specific expression

Specificity

Total Protein - Human Tumor Cell Line: Raji

Type

Cell

Tissue

Blood Lymph

Protein State

Native

Subcellular Fraction

Whole

Scientific Data Images for Raji Whole Cell Lysate

Western Blot: Raji Whole Cell Lysate [NB820-59466]

Western Blot: Raji Whole Cell Lysate [NB820-59466] - Protein lysate can be validated by Western Blot using specific biomarker: GAPDH for total protein, cytoplasmic and universal total protein; Histone for nuclear protein; Na/K ATPase for membrane protein; Vimentin for cytoskeleton protein.

SDS-PAGE: Raji Whole Cell Lysate [NB820-59466]

SDS-Page: Raji Whole Cell Lysate [NB820-59466] - Protein lysate shows consistent pattern.

Formulation, Preparation, and Storage

Formulation

HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors

Concentration

5.0 mg/ml

Shipping

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at -80C. Avoid freeze-thaw cycles.

Background: Raji

Tissue total protein is prepared from whole tissue homogenates and presents a consistent pattern on SDS-PAGE analysis.

Additional Raji Products

Product Documents for Raji Whole Cell Lysate

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Product Specific Notices for Raji Whole Cell Lysate

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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