Ran Antibody (8D1H12) - BSA Free

Novus Biologicals | Catalog # NBP2-61840

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Rat, Monkey

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 8D1H12

Format

BSA Free
View all Ran Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human RAN (AA: 1-216) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

24.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Ran Antibody (8D1H12) - BSA Free

Western Blot: Ran Antibody (8D1H12)BSA Free [NBP2-61840]

Western Blot: Ran Antibody (8D1H12)BSA Free [NBP2-61840]

Western Blot: Ran Antibody (8D1H12) [NBP2-61840] - Analysis using RAN mAb against HEK293 (1) and RAN (AA: 1-216)-hIgGFc transfected HEK293 (2) cell lysate.
Immunohistochemistry: Ran Antibody (8D1H12) - BSA Free [NBP2-61840]

Immunohistochemistry: Ran Antibody (8D1H12) - BSA Free [NBP2-61840]

Immunohistochemistry: Ran Antibody (8D1H12) [NBP2-61840] - Analysis of paraffin-embedded rectum cancer tissues using RAN mouse mAb with DAB staining.
Western Blot: Ran Antibody (8D1H12)BSA Free [NBP2-61840]

Western Blot: Ran Antibody (8D1H12)BSA Free [NBP2-61840]

Western Blot: Ran Antibody (8D1H12) [NBP2-61840] - Analysis using RAN mAb against human RAN (AA: 1-216) recombinant protein. (Expected MW is 50.4 kDa)
Western Blot: Ran Antibody (8D1H12)BSA Free [NBP2-61840]

Western Blot: Ran Antibody (8D1H12)BSA Free [NBP2-61840]

Western Blot: Ran Antibody (8D1H12) [NBP2-61840] - Analysis using RAN mouse mAb against A431 (1), C6 (2), Jurkat (3), Hela (4), COS7 (5), and Jurkat (6) cell lysate.
Immunohistochemistry: Ran Antibody (8D1H12) - BSA Free [NBP2-61840]

Immunohistochemistry: Ran Antibody (8D1H12) - BSA Free [NBP2-61840]

Immunohistochemistry: Ran Antibody (8D1H12) [NBP2-61840] - Analysis of paraffin-embedded esophageal cancer tissues using RAN mouse mAb with DAB staining.
ELISA: Ran Antibody (8D1H12) - BSA Free [NBP2-61840]

ELISA: Ran Antibody (8D1H12) - BSA Free [NBP2-61840]

ELISA: Ran Antibody (8D1H12) [NBP2-61840] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
ELISA: Ran Antibody (8D1H12) - BSA Free [NBP2-61840]

ELISA: Ran Antibody (8D1H12) - BSA Free [NBP2-61840]

ELISA: Ran Antibody (8D1H12) [NBP2-61840] - Analysis of Hela cells using RAN mouse mAb (green) and negative control (red).

Applications for Ran Antibody (8D1H12) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Flow Cytometry

1:10-1:1000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:200 - 1:1000

Western Blot

1:100-1:2000

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Ran

RAN (ras-related nuclear protein) is a small GTP binding protein belonging to the RAS superfamily that is essential for the translocation of RNA and proteins through the nuclear pore complex. The RAN protein is also involved in control of DNA synthesis and cell cycle progression. Nuclear localization of RAN requires the presence of regulator of chromosome condensation 1 (RCC1). Mutations in RAN disrupt DNA synthesis. Because of its many functions, it is likely that RAN interacts with several other proteins. RAN regulates formation and organization of the microtubule network independently of its role in the nucleus-cytosol exchange of macromolecules. RAN could be a key signaling molecule regulating microtubule polymerization during mitosis. RCC1 generates a high local concentration of RAN-GTP around chromatin which, in turn, induces the local nucleation of microtubules. RAN is an androgen receptor (AR) coactivator that binds differentially with different lengths of polyglutamine within the androgen receptor. Polyglutamine repeat expansion in the AR is linked to Kennedy's disease (X-linked spinal and bulbar muscular atrophy). RAN coactivation of the AR diminishes with polyglutamine expansion within the AR, and this weak coactivation may lead to partial androgen insensitivity during the development of Kennedy's disease.;

Alternate Names

Androgen receptor-associated protein 24, ARA24Gsp1, GTPase Ran, GTP-binding nuclear protein Ran, guanosine triphosphatase Ran, member RAS oncogene family, OK/SW-cl.81, RAN, member RAS oncogene family, RanGTPase, Ras-like protein TC4, Ras-related nuclear protein, TC4

Gene Symbol

RAN

Additional Ran Products

Product Documents for Ran Antibody (8D1H12) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Ran Antibody (8D1H12) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for Ran Antibody (8D1H12) - BSA Free

There are currently no reviews for this product. Be the first to review Ran Antibody (8D1H12) - BSA Free and earn rewards!

Have you used Ran Antibody (8D1H12) - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies