![Western Blot: Ran Antibody [NBP2-32281]](https://resources.rndsystems.com/images/products/Ran-Antibody-Western-Blot-NBP2-32281-img0001.jpg "Western Blot: Ran Antibody [NBP2-32281]")
Key Product Details
Validated by
Biological Validation
Species Reactivity
Human, Mouse
Applications
Immunohistochemistry, Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
The immunogen this antibody was made to, maps to a region between residue 166 to 216 of human Ras Related Nuclear Protein using the numbering given in entry NP_006316.1 GeneID 5901.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Ran Antibody - BSA Free
Western Blot: Ran Antibody [NBP2-32281]
Western Blot: Ran Antibody [NBP2-32281] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-RAN antibody NBP2-32281 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.![Immunohistochemistry: Ran Antibody [NBP2-32281]](https://resources.rndsystems.com/images/products/Ran-Antibody-Immunohistochemistry-NBP2-32281-img0004.jpg "Immunohistochemistry: Ran Antibody [NBP2-32281]")
Immunohistochemistry: Ran Antibody [NBP2-32281]
Immunohistochemistry: Ran Antibody [NBP2-32281] - Samples: FFPE serial sections of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti- RAN used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).![Immunohistochemistry: Ran Antibody [NBP2-32281]](https://resources.rndsystems.com/images/products/Ran-Antibody-Immunohistochemistry-NBP2-32281-img0003.jpg "Immunohistochemistry: Ran Antibody [NBP2-32281]")
Immunohistochemistry: Ran Antibody [NBP2-32281]
Immunohistochemistry: Ran Antibody [NBP2-32281] - Samples: FFPE serial sections of human breast carcinoma. Antibody: Affinity purified rabbit anti- RAN used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).![Immunoprecipitation: Ran Antibody [NBP2-32281]](https://resources.rndsystems.com/images/products/Ran-Antibody-Immunoprecipitation-NBP2-32281-img0002.jpg "Immunoprecipitation: Ran Antibody [NBP2-32281]")
Immunoprecipitation: Ran Antibody [NBP2-32281]
Immunoprecipitation: Ran Antibody [NBP2-32281] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-RAN antibody NBP2-32281 used for IP at 6 ug per reaction. For blotting immunoprecipitated RAN, NBP2-32281 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.Applications for Ran Antibody - BSA Free
Application
Recommended Usage
Immunoprecipitation
2 - 10 ug/mg lysate
Western Blot
1:2000 - 1:10000
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: Ran
Alternate Names
Androgen receptor-associated protein 24, ARA24Gsp1, GTPase Ran, GTP-binding nuclear protein Ran, guanosine triphosphatase Ran, member RAS oncogene family, OK/SW-cl.81, RAN, member RAS oncogene family, RanGTPase, Ras-like protein TC4, Ras-related nuclear protein, TC4
Gene Symbol
RAN
UniProt
Additional Ran Products
Product Documents for Ran Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Ran Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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