RanBP1 Antibody (HL2358) - Azide and BSA Free
Novus Biologicals | Catalog # NBP3-25621
Recombinant Monoclonal Antibody
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Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # HL2358 expressed in HEK293
Format
Azide and BSA Free
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Product Specifications
Immunogen
Recombinant fragmemt of human RanBP1.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for RanBP1 Antibody (HL2358) - Azide and BSA Free
Western Blot: RanBP1 Antibody (HL2358) - Azide and BSA Free [NBP3-25621] -
Western Blot: RanBP1 Antibody (HL2358) - Azide and BSA Free [NBP3-25621] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with RanBP1 antibody [HL2358] (NBP3-25621) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Western Blot: RanBP1 Antibody (HL2358) - Azide and BSA Free [NBP3-25621] -
Western Blot: RanBP1 Antibody (HL2358) - Azide and BSA Free [NBP3-25621] - Wild-type (WT) and RanBP1 knockout (KO) 293T cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with RanBP1 antibody [HL2358] (NBP3-25621) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunocytochemistry/Immunofluorescence: RanBP1 Antibody (HL2358) - Azide and BSA Free [NBP3-25621] -
Immunocytochemistry/Immunofluorescence: RanBP1 Antibody (HL2358) - Azide and BSA Free [NBP3-25621] - RanBP1 antibody [HL2358] detects RanBP1 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: RanBP1 stained by RanBP1 antibody [HL2358] (NBP3-25621) diluted at 1:500. Blue: Fluoroshield with DAPI.
Western Blot: RanBP1 Antibody (HL2358) - Azide and BSA Free [NBP3-25621] -
Western Blot: RanBP1 Antibody (HL2358) - Azide and BSA Free [NBP3-25621] - Various tissue extracts (50 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with RanBP1 antibody [HL2358] (NBP3-25621) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunohistochemistry-Paraffin: RanBP1 Antibody (HL2358) [NBP3-25621] -
RanBP1 antibody [HL2358] detects RanBP1 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded human colon cancer.
RanBP1 stained by RanBP1 antibody [HL2358] (NBP3-25621) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Applications for RanBP1 Antibody (HL2358) - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Western Blot
1:500-1:3000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: RanBP1
Alternate Names
HTF9A, MGC88701, RAN binding protein 1, Ran-binding protein 1, RanBP1, ran-specific GTPase-activating protein
Gene Symbol
RANBP1
Additional RanBP1 Products
Product Documents for RanBP1 Antibody (HL2358) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for RanBP1 Antibody (HL2358) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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