RanBP1 Antibody - BSA Free

Novus Biologicals | Catalog # NB100-79814

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all RanBP1 Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residues 1 and 50 of human RAN Binding Protein 1 using the numbering given in entry NP_002873.1 (GeneID 5902).

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 28821644).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RanBP1 Antibody - BSA Free

Western Blot: RanBP1 Antibody [NB100-79814]

Western Blot: RanBP1 Antibody [NB100-79814]

Western Blot: RanBP1 Antibody [NB100-79814] - Detection of Human RanBP1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RanBP1 antibody NB100-79814 used for WB at 0.2 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Western Blot: RanBP1 Antibody [NB100-79814]

Western Blot: RanBP1 Antibody [NB100-79814]

Western Blot: RanBP1 Antibody [NB100-79814] - Detection of Human RanBP1 on HeLa whole cell lysate using NB100-79814. RanBP1 was also immunoprecipitated using rabbit anti-RanBP1 antibody BL1968 NB100-79815 at 5 mcg/0.5 mg lysate.

Applications for RanBP1 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:100-1:2000

Immunohistochemistry-Paraffin

1:100-1:2000

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:1000-1:5000
Application Notes
IHC-P, IHC application reported in customer review.

Reviewed Applications

Read 2 reviews rated 5 using NB100-79814 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: RanBP1

Ran/TC4-binding protein, RanBP1, interacts specifically with GTP-charged RAN. RANBP1 encodes a 23-kD protein that binds to RAN complexed with GTP but not GDP. RANBP1 does not activate GTPase activity of RAN but does markedly increase GTP hydrolysis by the RanGTPase-activating protein (RanGAP1). The RANBP1 cDNA encodes a 201-amino acid protein that is 92% similar to its mouse homolog. In both mammalian cells and in yeast, RANBP1 acts as a negative regulator of RCC1 by inhibiting RCC1-stimulated guanine nucleotide release from RAN.

Alternate Names

HTF9A, MGC88701, RAN binding protein 1, Ran-binding protein 1, RanBP1, ran-specific GTPase-activating protein

Entrez Gene IDs

5902 (Human); 19385 (Mouse)

Gene Symbol

RANBP1

UniProt

NP_002873.1 (Human)

Additional RanBP1 Products

Product Documents for RanBP1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for RanBP1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for RanBP1 Antibody - BSA Free

Customer Reviews for RanBP1 Antibody - BSA Free (2)

5 out of 5
2 Customer Ratings
5 Stars
100%
4 Stars
0%
3 Stars
0%
2 Stars
0%
1 Stars
0%

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Customer Images

Showing  1 - 2 of 2 reviews Showing All
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  • Name: Anonymous
    Application: Immunofluorescence
    Sample Tested: HEK293
    Species: Human
    Verified Customer | Posted 04/16/2015
    RanBP1 Antibody - BSA Free NB100-79814
  • Name: Maja Stalekar
    Application: Immunohistochemistry-Paraffin
    Sample Tested: mouse spinal cord
    Species: Mouse
    Verified Customer | Posted 10/09/2013
    RanBP1 Antibody - BSA Free NB100-79814

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