RanBP16 Antibody (HL2510)
Novus Biologicals | Catalog # NBP3-48692
Recombinant Monoclonal Antibody
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Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Human, Mouse
Applications
Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # HL2510
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Product Specifications
Immunogen
Recombinant fragment of Human RanBP16.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for RanBP16 Antibody (HL2510)
Western Blot: RanBP16 Antibody (HL2510) [NBP3-48692] -
Non-transfected (-) and transfected (+) A549 whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with RanBP16 antibody [HL2510] (NBP3-48692) diluted at 1:9000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Western Blot: RanBP16 Antibody (HL2510) [NBP3-48692] -
Various tissue extracts (50 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with RanBP16 antibody [HL2510] (NBP3-48692) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Western Blot: RanBP16 Antibody (HL2510) [NBP3-48692] -
Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with RanBP16 antibody [HL2510] (NBP3-48692) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Immunocytochemistry/ Immunofluorescence: RanBP16 Antibody (HL2510) [NBP3-48692] -
RanBP16 antibody [HL2510] detects RanBP16 protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: A549 cells were fixed in 4% PFA.
Green: RanBP16 stained by RanBP16 antibody [HL2510] (NBP3-48692) diluted at 1:500.
Blue: Fluoroshield with DAPI.
Applications for RanBP16 Antibody (HL2510)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: RanBP16
Alternate Names
EXP7, exportin 7, KIAA0745exportin-7, RAN binding protein 16, Ran-binding protein 16, RANBP16Exp7
Gene Symbol
XPO7
Additional RanBP16 Products
Product Documents for RanBP16 Antibody (HL2510)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for RanBP16 Antibody (HL2510)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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