RASSF4 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-89249

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Porcine

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: QDDREQVHLPSTSWMPRRPSCPLKEPSPQNGNITAQGPSIQPVHKAESSTDSSGPLEEAEEAPQLMRTKSDASCMSQRRPKCRAPGEAQRI

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RASSF4 Antibody - BSA Free

Western Blot: RASSF4 Antibody [NBP1-89249]

Western Blot: RASSF4 Antibody [NBP1-89249]

Western Blot: RASSF4 Antibody [NBP1-89249] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue
Immunocytochemistry/ Immunofluorescence: RASSF4 Antibody [NBP1-89249]

Immunocytochemistry/ Immunofluorescence: RASSF4 Antibody [NBP1-89249]

Immunocytochemistry/Immunofluorescence: RASSF4 Antibody [NBP1-89249] - Staining of human cell line A-431 shows localization to nucleus, nucleoli & cell junctions. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: RASSF4 Antibody [NBP1-89249]

Immunohistochemistry-Paraffin: RASSF4 Antibody [NBP1-89249]

Immunohistochemistry-Paraffin: RASSF4 Antibody [NBP1-89249] - Staining of human fallopian tube shows moderate to strong nuclear positivity in glanuldar cells.
Immunohistochemistry-Paraffin: RASSF4 Antibody [NBP1-89249]

Immunohistochemistry-Paraffin: RASSF4 Antibody [NBP1-89249]

Immunohistochemistry-Paraffin: RASSF4 Antibody [NBP1-89249] - Staining of human stomach shows moderate to strong nuclear positivity in glandular cells.
Immunohistochemistry-Paraffin: RASSF4 Antibody [NBP1-89249]

Immunohistochemistry-Paraffin: RASSF4 Antibody [NBP1-89249]

Immunohistochemistry-Paraffin: RASSF4 Antibody [NBP1-89249] - Staining of human kidney shows strong nuclear postivity in glomeruli.
Immunohistochemistry-Paraffin: RASSF4 Antibody [NBP1-89249]

Immunohistochemistry-Paraffin: RASSF4 Antibody [NBP1-89249]

Immunohistochemistry-Paraffin: RASSF4 Antibody [NBP1-89249] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.

Applications for RASSF4 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200-1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RASSF4

The function of this gene has not yet been determined but may involve a role in tumor suppression. Alternative splicingof this gene results in several transcript variants; however, most of the variants have not been fully described.(provided by RefSeq)

Alternate Names

AD037, MGC44914, Ras association (RalGDS/AF-6) domain family 4, Ras association (RalGDS/AF-6) domain family member 4, Ras association domain family 4, ras association domain-containing protein 4, tumor suppressor RASSF4

Gene Symbol

RASSF4

Additional RASSF4 Products

Product Documents for RASSF4 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for RASSF4 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for RASSF4 Antibody - BSA Free

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Protocols

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FAQs

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