Introduction
R&D Systems Rat Adipokine Array (Catalog # ARY016) is a rapid, sensitive, and economical tool used to simultaneously detect the relative levels of 30 different obesity-related molecules in a single sample. No specialized equipment is necessary.
| Simultaneously detect the levels of these obesity-related proteins in a single sample. | ||
| ANGPT-L3 | IGFBP-1 | LIF |
| CCL2/MCP-1/JE | IGFBP-2 | Lipocalin-2/NGAL |
| CCL5/RANTES | IGFBP-3 | M-CSF |
| DPPIV/CD26 | IGFBP-5 | PAI-1/Serpin E1 |
| Endocan | IGFBP-6 | Pref-1 |
| FGF-21 | IL-1 beta | RAGE |
| HGF | IL-6 | Resistin |
| ICAM-1 | IL-10 | TIMP-1 |
| IGF-I | IL-11 | TNF-alpha |
| IGF-II | Leptin | VEGF |
General Assay Principle
Carefully selected capture antibodies have been spotted in duplicate on nitrocellulose membranes. Cell culture supernatant, cell lysate, tissue lysate, or serum samples are diluted and mixed with a cocktail of biotinylated detection antibodies. The sample/antibody mixture is then incubated with the array. Any cytokine/detection antibody complex present is bound by its cognate immobilized capture antibody on the membrane. Streptavidin-Horseradish Peroxidase and chemiluminescent detection reagents are added, and a signal is produced in proportion to the amount of cytokine bound. Chemiluminescence is detected in the same manner as a Western blot.
Kit Contents
- 4 Array Membranes
- 4-Well Multi-dish
- Array Buffers
- Wash Buffer
- Detection Antibody Cocktail
- Streptavidin-HRP
- Chemiluminescent Detection Reagents
- Transparency Overlay Template
- Detailed Protocol
For a complete list of the kit contents and necessary materials, please see the Materials Provided/Materials Required sections of the product datasheet.