Rat GM-CSF Quantikine ELISA Kit

Name: Rat GM-CSF Quantikine ELISA Kit
Brand: R&D Systems
SKU: RGM00

R&D Systems | Catalog # RGM00

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Product Documents

Discontinued Product

RGM00 has been discontinued. View all GM-CSF products.

Product Summary
Product Specifications
Precision
Recovery
Linearity
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 µL), Serum (50 µL)

Sensitivity

2.1 pg/mL

Assay Range

15.6-1000 pg/mL (Cell Culture Supernates, Serum)

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Product Summary for Rat GM-CSF Quantikine ELISA Kit

The Quantikine Rat GM-CSF Immunoassay is a 4.5 hour solid phase ELISA designed to measure rat GM-CSF in cell culture supernates and rat serum. It contains E. coli-expressed recombinant rat GM-CSF and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant rat GM-CSF. Results obtained using natural rat GM-CSF showed dose response curves that were parallel to the standard curves obtained using the recombinant rat kit standards. These results indicate that this kit can be used to determine relative mass values for natural rat GM-CSF.

Product Specifications

Measurement

Quantitative ELISA

Detection Method

Colorimetric - 450nm (TMB)

Species

Rat

Specificity

Natural and recombinant rat GM-CSF

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Cell Culture Supernates, Serum

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	55	176	746	56	172	723
Standard Deviation	4.6	6.9	30	5.5	10	43
CV%	8.4	3.9	4.0	9.8	5.8	5.9

Recovery for Rat GM-CSF Quantikine ELISA Kit

The recovery of rat GM-CSF spiked to three levels throughout the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=6)	97	89-107
Serum (n=14)	93	80-105

Linearity

To assess the linearity of the assay, six samples containing and/or spiked with high concentrations of rat GM-CSF in each matrix were diluted with Calibrator Diluent and then assayed.

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: GM-CSF

GM-CSF (Granulocyte-Macrophage Colony Stimulating Factor) induces the development of monocytes, neutrophils, eosinophils, and myeloid and dermal dendritic cells. It also acts as a neutrophil and dendritic cell chemoattractant. GM-CSF promotes Th1 and Th17 cell mediated autoimmune inflammation as well as the inflammatory activation of dendritic cells, microglia, alveolar macrophages, and eosinophils. In addition, it cooperates with G-CSF in promoting tumor cell proliferation and invasion. GM-CSF signals through a receptor complex composed of GM-CSF R alpha and the Common beta Chain (beta c) with Syndecan-2 as a potential co-receptor. The beta c subunit is shared by the receptor complexes for IL-3 and IL-5.