Rat IgG VisUCyte HRP Polymer Antibody

R&D Systems | Catalog # VC005

R&D Systems
Discontinued Product
VC005 has been discontinued. View all IgG products.

Key Product Details

Species Reactivity

Rat

Applications

Immunohistochemistry

Label

HRP Polymer

Antibody Source

Polyclonal Goat IgG
View all IgG Secondary Antibodies »

Product Specifications

Immunogen

Rat IgG

Specificity

Detects heavy ( gamma ) chains on rat IgG and light chains on all rat immunoglobulins, based on IEP. No cross-reactivity is observed with non-immunoglobulin rat serum proteins or IgG from human or mouse, based on IEP. VisUCyte™ HRP Polymer provides high sensitivity with low background in IHC.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Rat IgG VisUCyte HRP Polymer Antibody

Detection of HSP27 in Human Ovary.

Detection of HSP27 in Human Ovary.

HSP27 was detected in paraffin-embedded sections human ovary using Rat Anti-Human HSP27 Monoclonal Antibody (Catalog # MAB15801) with Anti-Rat IgG VisUCyte HRP Polymer Antibody (Catalog # VC005). Tissue was stained with DAB (brown color) and counterstained with hematoxylin (blue color). View our protocol for theVisUCyte HRP Polymer Detection Reagent.

Applications for Rat IgG VisUCyte HRP Polymer Antibody

Application
Recommended Usage

Immunohistochemistry

Prediluted, ready to use with formalin fixed paraffin-embedded and frozen sections. Compatible with most commonly used fixatives.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

Supplied as a solution in PBS with preservative.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

6 months from date of receipt, 2 to 8 °C as supplied.

Background: IgG

VisUCyte™ HRP Polymer is a biotin-free detection reagent which overcomes problems of avidin-biotin detection chemistry, such as endogenous biotin staining and the need to do additional quenching of endogenous biotin and avidin present in some tissues. With VisUCyte HRP Polymer it is possible to achieve specific staining in cells and tissue much faster than the conventional avidin-biotin-HRP procedure. Due the high sensitivity of VisUCyte™ HRP Polymer, the working concentration of primary antibodies may be 2-3 times lower compared to the conventional avidin-biotin-HRP protocol.

Long Name

Immunoglobulin G

Alternate Names

Immunoglobulin G, ImmunoglobulinG

Additional IgG Products

Product Documents for Rat IgG VisUCyte HRP Polymer Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Rat IgG VisUCyte HRP Polymer Antibody

For research use only

Citations for Rat IgG VisUCyte HRP Polymer Antibody

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Protocols

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FAQs

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