Rat Neuropilin-2 Alexa Fluor® 488-conjugated Antibody

Discontinued Product

FAB567G has been discontinued.
Detection of Neuropilin‑2 in C6 Rat Cell Line by Flow Cytometry.
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Rat Neuropilin-2 Alexa Fluor® 488-conjugated Antibody Summary

Species Reactivity
Rat
Specificity
Detects rat Neuropilin-2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody does not cross-react with recombinant human Neuropilin‑1.
Source
Monoclonal Mouse IgG2B Clone # 96009
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant rat Neuropilin‑2
Gln23-Asp857 (Val809-Asp825 del)
Accession # O35276
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 488

Applications

Recommended Concentration
Sample
Flow Cytometry
5 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of Neuropilin‑2 in C6 Rat Cell Line by Flow Cytometry. View Larger

Detection of Neuropilin‑2 in C6 Rat Cell Line by Flow Cytometry. C6 rat glioma cell line was stained with Mouse Anti-Rat Neuropilin‑2 Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # FAB567G, filled histogram) or isotype control antibody (Catalog # IC0041G, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Neuropilin-2

 Neuropilin-2 (Npn-2), previously known as Npn-1-related molecule, is a 120-140 kDa type I transmembrane protein that belongs to the neuropilin family of molecules. It binds members of the class III secreted semaphorin subfamily (Sema3C and 3F), multiple VEGFs (VEGF145, VEGF165, VEGF-C and VEGF-D), PlGF, and HGF, and forms complexes with NRP1, Plexin A1,A2, A3, A4 and Plexin B1, plus VEGFR1, 2 and 3, and Integrin alpha 6 beta 1. The extracellular domain of these proteins is composed of two N-terminal CUB (complement-binding) domains (domains a1 and a2), two domains with homology to coagulation factors V and VIII (domains b1 and b2) and a MAM (meprin) domain (domain c). In the absence of ligands, neuropilins can form homo- and hetero-oligomers via homophilic interactions of their MAM domains. At the amino acid (aa) sequence level, Npn-2 shares 96% and 94% aa sequence identity with mouse and human Neuropilin-2, respectively. The expression patterns of Npn-1 and Npn-2 in developing neurons of the central and peripheral nervous systems are largely, though not completely non-overlapping. Npn-2 is expressed by vascular and lymphatic endothelim plus tumor cells, and has been suggested to appear also on macrophages, GABAergic neurons and proximal convoluted tubule cells.

Entrez Gene IDs
8828 (Human); 18187 (Mouse); 81527 (Rat)
Alternate Names
MGC126574; neuropilin 2; Neuropilin2; Neuropilin-2; neuropilin-2a(22); neuropilin-2b(0); NP2; NPN 2; NPN2; PRO2714; receptor for VEGF165 and semaphorins class3; Vascular endothelial cell growth factor 165 receptor 2; vascular endothelial growth factor-165 receptor 2; VEGF1265R2; VEGF165R2neuropilin-2a(17)

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