Key Product Details

Species Reactivity

Rat

Applications

Flow Cytometry

Label

Alexa Fluor 700 (Excitation = 675-700 nm, Emission = 723 nm)

Antibody Source

Recombinant Monoclonal Mouse IgG2B Clone # 96009R
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Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant rat Neuropilin‑2
Gln23-Asp857 (Val809-Asp825 del)
Accession # O35276

Specificity

Detects rat Neuropilin-2 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Applications for Rat Neuropilin‑2 Alexa Fluor® 700‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

0.25-1 µg/106 cells
Sample: C6 Rat Cell Line

Spectra Viewer

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Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Advanced Features

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Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Neuropilin-2

Neuropilin-1 (Npn-1, previously known as neuropilin) and Npn-2 (previously known as Npn-1-related molecule) are type I transmembrane proteins that bind members of the class III secreted semaphorin subfamily which are implicated in repulsive axon guidance. The extracellular domain of these proteins is composed of two N-terminal CUB (complement-binding) domains (domains a1 and a2), two domains with homology to coagulation factors V and VIII (domains b1 and b2) and a MAM (meprin) domain (domain c). In the absence of ligands, neuropilins can form homo- and hetero-oligomers via homophilic interactions of their MAM domains. At the amino acid sequence level, Npn-1and Npn-2 share 44% identity. Npn-1 and Npn-2 show different binding specificities for different members of the semaphorin family. The expression patterns of Npn-1 and Npn-2 in developing neurons of the central and peripheral nervous systems are largely, though not completely non-overlapping. Npn‑1 and Npn-2 are also expressed by endothelial and tumor cells and have been shown to be isoform-specific receptors for VEGF165. Npn-1 was also reported to bind PlGF-2 and the VEGF-like protein from of virus NZ2.

References

  1. Fujisawa, H. and T. Kitsukawa (1998) Curr. Opin. Neurobiol. 8:587.
  2. Neufeld, G. et al. (1999) FASEB J. 13:9.
  3. Poltorak, Z. et al. (2000) J. Biol. Chem. 275:18040.

Alternate Names

Neuropilin2

Entrez Gene IDs

8828 (Human); 18187 (Mouse); 81527 (Rat)

Gene Symbol

NRP2

UniProt

Additional Neuropilin-2 Products

Product Documents for Rat Neuropilin‑2 Alexa Fluor® 700‑conjugated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Rat Neuropilin‑2 Alexa Fluor® 700‑conjugated Antibody


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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Associated Pathways

VEGF - VEGF R2 Signaling Pathways VEGF - VEGF R2 Signaling Pathway Thumbnail