Rat Neuropilin-2 Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
Detection of Rat Neuropilin‑2 by Western Blot.
3 Images
Product Details
Supplemental Products

Rat Neuropilin-2 Antibody Summary

Species Reactivity
Detects rat Neuropilin-2 in direct ELISAs.
Recombinant Monoclonal Mouse IgG2B Clone # 96009R
Protein A or G purified from cell culture supernatant
Mouse myeloma cell line NS0-derived recombinant rat Neuropilin‑2
Gln23-Asp857 (Val809-Asp825 del)
Accession # O35276
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Complete Your Experiment


Recommended Concentration
Western Blot
1 µg/mL
See below
Simple Western
50 µg/mL
See below
Flow Cytometry
0.25 µg/106 cells
See below
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Rat Neuropilin-2 antibody by Western Blot. View Larger

Detection of Rat Neuropilin‑2 by Western Blot. Western blot shows lysates of C6 rat glioma cell line and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Rat Neuropilin-2 Monoclonal Antibody (Catalog # MAB567R) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Neuropilin-2 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Flow Cytometry Detection of Neuropilin-2 antibody in C6 Rat Cell Line antibody by Flow Cytometry. View Larger

Detection of Neuropilin-2 in C6 Rat Cell Line by Flow Cytometry. C6 Rat glial tumor cell line was stained with Mouse Anti-Rat Neuropilin-2 Monoclonal Antibody (Catalog # MAB567R, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Simple Western Detection of Rat Neuropilin-2 antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Rat Neuropilin‑2 by Simple WesternTM. Simple Western lane view shows lysates of C6 rat glioma cell line, loaded at 0.2 mg/mL. A specific band was detected for Neuropilin‑2 at approximately 138 kDa (as indicated) using 50 µg/mL of Mouse Anti-Rat Neuropilin‑2 Monoclonal Antibody (Catalog # MAB567R). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Neuropilin-2

Neuropilin-1 (Npn-1, previously known as neuropilin) and Npn-2 (previously known as Npn-1-related molecule) are type I transmembrane proteins that bind members of the class III secreted semaphorin subfamily which are implicated in repulsive axon guidance. The extracellular domain of these proteins is composed of two N-terminal CUB (complement-binding) domains (domains a1 and a2), two domains with homology to coagulation factors V and VIII (domains b1 and b2) and a MAM (meprin) domain (domain c). In the absence of ligands, neuropilins can form homo- and hetero-oligomers via homophilic interactions of their MAM domains. At the amino acid sequence level, Npn-1and Npn-2 share 44% identity. Npn-1 and Npn-2 show different binding specificities for different members of the semaphorin family. The expression patterns of Npn-1 and Npn-2 in developing neurons of the central and peripheral nervous systems are largely, though not completely non-overlapping. Npn‑1 and Npn-2 are also expressed by endothelial and tumor cells and have been shown to be isoform-specific receptors for VEGF165. Npn-1 was also reported to bind PlGF-2 and the VEGF-like protein from of virus NZ2.

  1. Fujisawa, H. and T. Kitsukawa (1998) Curr. Opin. Neurobiol. 8:587.
  2. Neufeld, G. et al. (1999) FASEB J. 13:9.
  3. Poltorak, Z. et al. (2000) J. Biol. Chem. 275:18040.
Entrez Gene IDs
8828 (Human); 18187 (Mouse); 81527 (Rat)
Alternate Names
MGC126574; neuropilin 2; Neuropilin2; Neuropilin-2; neuropilin-2a(22); neuropilin-2b(0); NP2; NPN 2; NPN2; PRO2714; receptor for VEGF165 and semaphorins class3; Vascular endothelial cell growth factor 165 receptor 2; vascular endothelial growth factor-165 receptor 2; VEGF1265R2; VEGF165R2neuropilin-2a(17)

Product Datasheets

You must select a language.



No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Rat Neuropilin-2 Antibody

There are currently no reviews for this product. Be the first to review Rat Neuropilin-2 Antibody and earn rewards!

Have you used Rat Neuropilin-2 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review