RB1 [p Ser780] Antibody (JE44-61)

Western Blot: RB1 [p Ser780] Antibody (JE44-61) [NBP3-32794]

Western Blot: RB1 [p Ser780] Antibody (JE44-61) [NBP3-32794] - Western blot analysis of RB1 on different lysates with Rabbit anti-RB1 antibody (NBP3-32794) at 1/1,000 dilution.

Lane 1: Jurkat serum starved for 44 hours whole cell lysate
Lane 2: Jurkat serum starved for 24 hours then 10% FBS incubated for 20 hours whole cell lysate
Lane 3: Jurkat serum starved for 44 hours then treated with pp for 1 hour whole cell lysate
Lane 4: Jurkat serum starved for 24 hours then 10% FBS incubated for 20 hours then treated with pp for 1 hour whole cell lysate

Lysates/proteins at 20 ug/Lane.

Predicted band size: 106 kDa
Observed band size: 106 kDa

Exposure time: 1 minute 59 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32794) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.