Recombinant Human RBBP4/RbAp48 Protein

Novus Biologicals | Catalog # NBP3-18203

Novus Biologicals
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Key Product Details

Source

Sf 9 (baculovirus)

Applications

Bioactivity, SDS-PAGE
View all RBBP4 Proteins and Enzymes »

Product Specifications

Description

A recombinant protein corresponding to the amino acids Met1-Ser425 of Human RBBP4/RbAp48.

Source: Sf 9 (baculovirus)

(Accession #: NP_005601.1)

Store at -20C to -80C long term. After reconstitution, the product is stable at -20C for 3 months, and 2-8C for up to 1 week.

Purity

>92%, by SDS-PAGE

Endotoxin Level

< 1.0 EU/ug of the protein by LAL method

Activity

Measured by its binding ability in a functional ELISA. Immobilized Human RBBP4/RbAp48 Protein at 1 ug/ml (100 ul/well) can bind RBBP4/RbAp48 Rabbit pAb with a linear range of 0.976-7.09 ng/ml.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human RBBP4/RbAp48 Protein

SDS-PAGE: Recombinant Human RBBP4/RbAp48 Protein [NBP3-18203]

SDS-PAGE: Recombinant Human RBBP4/RbAp48 Protein [NBP3-18203]

SDS-Page: Recombinant Human RBBP4/RbAp48 Protein [NBP3-18203] - Recombinant Human RBBP4/RbAp48 Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at approximately 50 kDa.

Formulation, Preparation, and Storage

NBP3-18203
Formulation Lyophilized from a 0.22 um filtered solution of 50mM Tris, 500mM NaCl, pH 7.4
Concentration Lyoph
Reconstitution Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at -20C.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: RBBP4

RbAp48 encodes a ubiquitously expressed nuclear protein which belongs to a highly conserved subfamily of WD-repeat proteins. It is present in protein complexes involved in histone acetylation and chromatin assembly. It is part of the Mi-2 complex which has been implicated in chromatin remodeling and transcriptional repression associated with histone deacetylation. This encoded protein is also part of co-repressor complexes, which is an integral component of transcriptional silencing. It is found among several cellular proteins that bind directly to retinoblastoma protein to regulate cell proliferation. This protein also seems to be involved in transcriptional repression of E2F-responsive genes.

Long Name

Retinoblastoma Binding Protein 4

Alternate Names

CAF-1 p48, CAF-1C, NURF55, RBAP48

Gene Symbol

RBBP4

Additional RBBP4 Products

Product Documents for Recombinant Human RBBP4/RbAp48 Protein

Certificate of Analysis

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Product Specific Notices for Recombinant Human RBBP4/RbAp48 Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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