RBM15B Antibody (A10D12+A10D11)

Western Blot: RBM15B Antibody (A10D12+A10D11) [NBP3-32898]

Western Blot: RBM15B Antibody (A10D12+A10D11) [NBP3-32898] - Western blot analysis of RBM15B on different lysates with Mouse anti-RBM15B antibody (NBP3-32898) at 1/2,000 dilution.

Lane 1: HeLa cell lysate
Lane 2: 293T cell lysate
Lane 3: Jurkat cell lysate
Lane 4: RAW264.7 cell lysate
Lane 5: NIH/3T3 cell lysate
Lane 6: RAW264.7 cell lysate
Lane 7: PC-12 cell lysate

Lysates/proteins at 30 ug/Lane.

Predicted band size: 97 kDa
Observed band size: 100 kDa

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 27602518). The bands below 100 kDa may be caused by degradation.

Exposure time: 1 minute 21 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32898) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.