RDH5 Antibody

Novus Biologicals | Catalog # NBP1-52047

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all RDH5 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-RDRQSLPASNAFV corresponding to internal region according to NP_002896.2.

Epitope

C-RDRQSLPASNAFV

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for RDH5 Antibody

RDH5 Antibody

Immunohistochemistry-Paraffin: RDH5 Antibody [NBP1-52047] -

Immunohistochemistry-Paraffin: RDH5 Antibody [NBP1-52047] - (5ug/ml) staining of paraffin embedded Human Retina. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
RDH5 Antibody

Immunocytochemistry/Immunofluorescence: RDH5 Antibody [NBP1-52047] -

Immunocytochemistry/Immunofluorescence: RDH5 Antibody [NBP1-52047] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing strong Endoplasmic Reticulum and weak cytoplasmic and nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
RDH5 Antibody

Flow Cytometry: RDH5 Antibody [NBP1-52047] -

Flow Cytometry: RDH5 Antibody [NBP1-52047] - Flow cytometric analysis of paraformaldehyde fixed U2OS cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Applications for RDH5 Antibody

Application
Recommended Usage

Flow Cytometry

10 ug/ml

Immunocytochemistry/ Immunofluorescence

10 ug/ml

Immunohistochemistry

5-10 ug/ml

Immunohistochemistry-Paraffin

5-10 ug/ml

Peptide ELISA

Detection limit 1:8000
Application Notes
WB: Preliminary testing showed a band at approx. 32kDa in Human Heart and approx. 35kDa in Human Breast lysate, after 0.3ug/ml antibody staining. (calculated MW of 35.0kDa according to NP_002896.2). Primary incubation 1 hour at room temperature. IHC-P: Human Retina shows staining of the ganglion cell layer.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: RDH5

This gene encodes an enzyme belonging to the short-chain dehydrogenases/reductases (SDR) family. This retinol dehydrogenase functions to catalyze the final step in the biosynthesis of 11-cis retinaldehyde, which is the universal chromophore of visual pigments. Mutations in this gene cause autosomal recessive fundus albipunctatus, a rare form of night blindness that is characterized by a delay in the regeneration of cone and rod photopigments. [provided by RefSeq]

Alternate Names

11-cis RDH, 11-cis retinol dehydrogenase, 9,9-cis-retinol specific dehydrogenase, EC 1.1.1, EC 1.1.1.105, FLJ39337, FLJ97089, HSD17B, RDH1, retinol dehydrogenase 1, retinol dehydrogenase 5 (11-cis and 9-cis), retinol dehydrogenase 5 (11-cis/9-cis), SDR9C5, short chain dehydrogenase/reductase family 9C, member 5

Entrez Gene IDs

5959 (Human); 19682 (Mouse)

Gene Symbol

RDH5

UniProt

NP_002896.2 (Human)

Additional RDH5 Products

Product Documents for RDH5 Antibody

Certificate of Analysis

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Product Specific Notices for RDH5 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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