Recombinant A. caninum NIF Protein, CF

R&D Systems | Catalog # 5845-NF

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Key Product Details

  • R&D Systems NS0-derived Recombinant A. caninum NIF Protein (5845-NF)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

NS0

Accession Number

AAA27789

Applications

Bioactivity
View all NIF Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived a. caninum NIF protein
Asn18-Leu274, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Asn18

Predicted Molecular Mass

30 kDa

SDS-PAGE

55-65 kDa, reducing conditions

Activity

Measured by its ability to inhibit the adhesion of PMA-activated human neutrophils.
The ED50 for this effect is 0.1-0.4 μg/mL.

Also measured by its ability to bind Recombinant Human Integrin  alpha M beta 2 (Catalog # 4047-AM) in a functional ELISA.

Reviewed Applications

Read 1 review rated 5 using 5845-NF in the following applications:

Formulation, Preparation, and Storage

5845-NF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS, NaCl, and DTT.
Reconstitution

Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: NIF

Neutrophil inhibitory factor (NIF) of the dog hookworm, Ancylostoma caninum, is a 41 kDa secreted glycoprotein that is thought to allow the hookworm to evade the innate immune defenses of the host (1, 2). NIF interacts with the leukocyte integrin alpha M beta 2 (CD11b/CD18, also called Mac-1), thus inhibiting mammalian neutrophil adhesion to endothelium (1). The A. caninum NIF cDNA encodes 257 amino acids (aa) including a 17 aa signal sequence and a 240 aa mature protein with 10 cysteine residues and 7 potential N-linked glycosylation sites (1). Incubation of mammalian neutrophils with NIF does not appear to be toxic, but does result in rapid, cation‑dependent, reversible inhibition of alpha M beta 2-mediated adhesion and degranulation (1 - 4). The NIF binding site within the alpha M beta 2 I-domain interferes with activation‑dependent adhesion sites of ligands including ICAM-1/CD54, complement component C3b, and fibrinogen (2, 3). In mouse or guinea pig inflammatory lung injury models, administration of NIF prevented neutrophil-dependent vascular injury (4, 5).

References

  1. Moyle, M. et al. (1994) J. Biol. Chem. 269:10008.
  2. Rieu, P. et al. (1994) J. Cell Biol. 127:2081.
  3. Ustinov, V.A. and E.F. Plow (2002) J. Biol. Chem. 277:18769.
  4. Barnard, J.W. et al. (1995) J. Immunol. 155:4876.
  5. Zhou, M.-Y. et al. (1998) J. Clin. Invest. 101:2427.

Long Name

Neutrophil Inhibitory Factor

UniProt

AAA27789 (A. caninum)

Additional NIF Products

Product Documents for Recombinant A. caninum NIF Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Recombinant A. caninum NIF Protein, CF

For research use only

Related Research Areas

Integrins and Associated Molecules

Citations for Recombinant A. caninum NIF Protein, CF

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