Recombinant A. caninum NIF Protein, CF

Catalog # Availability Size / Price Qty
5845-NF-050
Product Details
Citations (2)
FAQs
Supplemental Products
Reviews (1)

Recombinant A. caninum NIF Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit the adhesion of PMA-activated human neutrophils. The ED50 for this effect is 0.1-0.4 μg/mL. Also measured by its ability to bind Recombinant Human Integrin  alpha M beta 2 (Catalog # 4047-AM) in a functional ELISA.
Source
Mouse myeloma cell line, NS0-derived a. caninum NIF protein
Asn18-Leu274, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Asn18
Predicted Molecular Mass
30 kDa
SDS-PAGE
55-65 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

5845-NF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS, NaCl, and DTT.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: NIF

Neutrophil inhibitory factor (NIF) of the dog hookworm, Ancylostoma caninum, is a 41 kDa secreted glycoprotein that is thought to allow the hookworm to evade the innate immune defenses of the host (1, 2). NIF interacts with the leukocyte integrin alpha M beta 2 (CD11b/CD18, also called Mac-1), thus inhibiting mammalian neutrophil adhesion to endothelium (1). The A. caninum NIF cDNA encodes 257 amino acids (aa) including a 17 aa signal sequence and a 240 aa mature protein with 10 cysteine residues and 7 potential N-linked glycosylation sites (1). Incubation of mammalian neutrophils with NIF does not appear to be toxic, but does result in rapid, cation‑dependent, reversible inhibition of alpha M beta 2-mediated adhesion and degranulation (1 - 4). The NIF binding site within the alpha M beta 2 I-domain interferes with activation‑dependent adhesion sites of ligands including ICAM-1/CD54, complement component C3b, and fibrinogen (2, 3). In mouse or guinea pig inflammatory lung injury models, administration of NIF prevented neutrophil-dependent vascular injury (4, 5).

References
  1. Moyle, M. et al. (1994) J. Biol. Chem. 269:10008.
  2. Rieu, P. et al. (1994) J. Cell Biol. 127:2081.
  3. Ustinov, V.A. and E.F. Plow (2002) J. Biol. Chem. 277:18769.
  4. Barnard, J.W. et al. (1995) J. Immunol. 155:4876.
  5. Zhou, M.-Y. et al. (1998) J. Clin. Invest. 101:2427.
Long Name
Neutrophil Inhibitory Factor
Alternate Names
NIF

Citations for Recombinant A. caninum NIF Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
Filter your results:

Filter by:

  1. A ligand-specific blockade of the integrin Mac-1 selectively targets pathologic inflammation while maintaining protective host-defense
    Authors: D Wolf, N Anto-Miche, H Blankenbac, A Wiedemann, K Buscher, JD Hohmann, B Lim, M Bäuml, A Marki, M Mauler, D Duerschmie, Z Fan, H Winkels, D Sidler, P Diehl, DM Zajonc, I Hilgendorf, P Stachon, T Marchini, F Willecke, M Schell, B Sommer, C von Zur Mu, J Reinöhl, T Gerhardt, EF Plow, V Yakubenko, P Libby, C Bode, K Ley, K Peter, A Zirlik
    Nat Commun, 2018;9(1):525.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Microglial dopamine receptor elimination defines sex-specific nucleus accumbens development and social behavior in adolescent rats
    Authors: AM Kopec, CJ Smith, NR Ayre, SC Sweat, SD Bilbo
    Nat Commun, 2018;9(1):3769.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo

FAQs

No product specific FAQs exist for this product, however you may

View all Proteins and Enzyme FAQs

Recombinant Proteins

Reconstitution Buffers

Reviews for Recombinant A. caninum NIF Protein, CF

Average Rating: 5 (Based on 1 Review)

5 Star
100%
4 Star
0%
3 Star
0%
2 Star
0%
1 Star
0%

Have you used Recombinant A. caninum NIF Protein, CF?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:


Recombinant A. caninum NIF Protein, CF
By Anonymous on 09/14/2019
Application: SDS-PAGE Control