Recombinant A. caninum NIF Protein, CF
Recombinant A. caninum NIF Protein, CF Summary
Asn18-Leu274, with a C-terminal 6-His tag
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS, NaCl, and DTT.|
|Reconstitution||Reconstitute at 200 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Neutrophil inhibitory factor (NIF) of the dog hookworm, Ancylostoma caninum, is a 41 kDa secreted glycoprotein that is thought to allow the hookworm to evade the innate immune defenses of the host (1, 2). NIF interacts with the leukocyte integrin alpha M beta 2 (CD11b/CD18, also called Mac-1), thus inhibiting mammalian neutrophil adhesion to endothelium (1). The A. caninum NIF cDNA encodes 257 amino acids (aa) including a 17 aa signal sequence and a 240 aa mature protein with 10 cysteine residues and 7 potential N-linked glycosylation sites (1). Incubation of mammalian neutrophils with NIF does not appear to be toxic, but does result in rapid, cation‑dependent, reversible inhibition of alpha M beta 2-mediated adhesion and degranulation (1 - 4). The NIF binding site within the alpha M beta 2 I-domain interferes with activation‑dependent adhesion sites of ligands including ICAM-1/CD54, complement component C3b, and fibrinogen (2, 3). In mouse or guinea pig inflammatory lung injury models, administration of NIF prevented neutrophil-dependent vascular injury (4, 5).
- Moyle, M. et al. (1994) J. Biol. Chem. 269:10008.
- Rieu, P. et al. (1994) J. Cell Biol. 127:2081.
- Ustinov, V.A. and E.F. Plow (2002) J. Biol. Chem. 277:18769.
- Barnard, J.W. et al. (1995) J. Immunol. 155:4876.
- Zhou, M.-Y. et al. (1998) J. Clin. Invest. 101:2427.
Citations for Recombinant A. caninum NIF Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 2
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A ligand-specific blockade of the integrin Mac-1 selectively targets pathologic inflammation while maintaining protective host-defense
Authors: D Wolf, N Anto-Miche, H Blankenbac, A Wiedemann, K Buscher, JD Hohmann, B Lim, M Bäuml, A Marki, M Mauler, D Duerschmie, Z Fan, H Winkels, D Sidler, P Diehl, DM Zajonc, I Hilgendorf, P Stachon, T Marchini, F Willecke, M Schell, B Sommer, C von Zur Mu, J Reinöhl, T Gerhardt, EF Plow, V Yakubenko, P Libby, C Bode, K Ley, K Peter, A Zirlik
Nat Commun, 2018;9(1):525.
Sample Types: Whole Cells
Microglial dopamine receptor elimination defines sex-specific nucleus accumbens development and social behavior in adolescent rats
Authors: AM Kopec, CJ Smith, NR Ayre, SC Sweat, SD Bilbo
Nat Commun, 2018;9(1):3769.
Sample Types: In Vivo
Applications: In Vivo
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