Recombinant Human Integrin alpha M beta 2 Protein, CF

R&D Systems | Catalog # 4047-AM

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Key Product Details

  • R&D Systems CHO-derived Recombinant Human Integrin alpha M beta 2 Protein (4047-AM)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

CHO

Accession Number

NP_001139280 (Integrin alpha M) & P05107 (Integrin beta 2)

Applications

Bioactivity
View all Integrin alpha M beta 2 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human Integrin alpha M beta 2 protein
Human Integrin alpha M
(Phe17-Asn1105)
Accession # NP_001139280		 acidic tail
Human Integrin beta 2
(Gln23-Asn700)
Accession # P05107		 basic tail
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Phe17 (Integrin alpha M) & No results obtained: Gln23 predicted (Integrin beta 2)

Predicted Molecular Mass

124.7 kDa (Integrin alpha M), 79 kDa (Integrin beta 2)

SDS-PAGE

165 kDa and 100 kDa, reducing conditions

Activity

Measured by the ability of the immobilized protein to support the adhesion of CHO Chinese hamster ovary cells transfected with ICAM-1.
The ED50 for this effect is 0.25-1.5 μg/mL.

Formulation, Preparation, and Storage

4047-AM
Formulation Lyophilized from a 0.2 μm filtered solution in Tris, NaCl and MgCl2.
Reconstitution

Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Integrin alpha M beta 2

Integrin alpha M beta 2, also called MAC-1 or complement receptor type 3 (CR3), is one of three leukocyte beta 2 integrins. The non-covalent heterodimer of 170 kDa alpha M/CD11b and 95 kDa beta 2/CD18 integrin subunits is expressed mainly on myeloid and natural killer cells (1-6). The alpha M vWFA or I-domain, which contains adhesion sites, forms the N-terminal head region with the alpha M beta -propeller and the beta 2 vWFA domain. Unlike most integrins, the calf domain of alpha M is lectin-like and binds carbohydrates (7). Each subunit has a transmembrane sequence and a short cytoplasmic tail. The 1088 amino acid (aa) human alpha M/CD11b ECD shares 73-76% aa sequence identity with mouse, rat, bovine, and canine alpha M, while the 678 aa human beta 2/CD18 ECD shares 81 - 83% aa sequence identity with mouse, rat, bovine, canine, goat, sheep, and porcine beta 2. Like other integrins, alpha M beta 2 has multiple activation states (1-3). In the presence of divalent cations and "inside-out" signaling, alpha M beta 2 is fully active and extended. In the inactive state, the heterodimer flexes in the center at the alpha M thigh and calf domains and beta 2 I-EGF domains, impeding access to adhesion sites. Active alpha M beta 2 binds an unusually large number of adhesion partners, including the complement opsonin fragment iC3b, coagulation proteins fibrinogen, plasminogen and factor X, extracellular matrix (ECM) proteins fibronectin, laminin and collagen, and cell surface ICAMs, myelin basic protein and DC-SIGN (3, 4, 7). alpha M beta 2 lectin-like adhesion partners include heparin, bacterial lipopolysaccharides, and GPI-linked glycoproteins such as uPAR and Fc gamma RIIIB (3, 7). Binding of platelet JAM-C links platelets with myeloid and dendritic cell (DC) alpha M beta 2 and recruits these cells to inflamed or injured endothelium, while neutrophil alpha M beta 2 adheres to RAGE on inflamed endothelium; both are atherogenic events (3, 8, 9). However, activation of alpha M beta 2 inhibits alternative activation of macrophages and atherosclerotic foam cell formation (3, 10). alpha M beta 2 can either suppress or allow constitutive neutrophil apoptosis, depending on its ligand and activation state (3, 11, 12). Deletion of mouse alpha M causes defects in neutrophil adhesion and degranulation, while mutations of human or mouse beta 2 cause leukocyte adhesion deficiency (LAD-1) and susceptibility to bacterial infections (3, 12, 13).

References

  1. Takada, Y. et al. (2007) Genome Biol. 8:215.
  2. Luo, B-H. et al. (2007) Annu. Rev. Immunol. 25:619.
  3. Tan, S.M. (2012) Biosci. Rep. 32:241.
  4. Corbi, A.L. et al. (1988) J. Biol. Chem. 263:12403.
  5. Kishimoto, T. K. et al. (1987) Cell 48:681.
  6. Muto, S. et al. (1993) J. Clin. Immunol. 13:175.
  7. Xia, Y. et al. (2002) J. Immunol. 169:6417.
  8. Santoso, S. et al. (2002) J. Exp. Med. 196:679.
  9. Langer, H.F. et al. (2007) Arterioscler. Thromb. Vasc. Biol. 27:1463.
  10. Yakubenko, V.P. et al. (2011) Circ. Res. 108:544.
  11. Pluskota, E. et al. (2008) J. Immunol. 181:3609.
  12. Coxon, A. et al. (1996) Immunity 5:653.
  13. Lu, H. et al. (1997) J. Clin. Invest. 99:1340.

Entrez Gene IDs

3684 (Human)

Gene Symbol

ITGAM

UniProt

NP_001139280 (Integrin alpha M) & P05107 (Integrin beta 2)

Additional Integrin alpha M beta 2 Products

Product Documents for Recombinant Human Integrin alpha M beta 2 Protein, CF

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Product Specific Notices for Recombinant Human Integrin alpha M beta 2 Protein, CF

For research use only

Citations for Recombinant Human Integrin alpha M beta 2 Protein, CF

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FAQs for Recombinant Human Integrin alpha M beta 2 Protein, CF

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  • Q: What is the amino acid sequence of the acidic and basic tails?

    A: Acidic and basic tails are added to the protein to help facilitate optimal activity. While we generally include sequence information on the product datasheet, the sequences of these tails are considered confidential information.

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