Recombinant BatCoV RaTG13 Spike (GCN4-IZ) His Protein, CF

R&D Systems | Catalog # 10688-CV

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Key Product Details

  • R&D Systems HEK293-derived Recombinant BatCoV RaTG13 Spike (GCN4-IZ) His Protein (10688-CV)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

HEK293

Accession Number

QHR63300.2

Applications

Bioactivity
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Product Specifications

Source

Human embryonic kidney cell, HEK293-derived batcov ratg13 Spike protein
BatCoV RaTG13 Spike
(Val16-Pro1209)
Accession # QHR63300.2		 GCN4-IZ HHHHHH
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Val16

Predicted Molecular Mass

138 kDa

SDS-PAGE

140-160 kDa, under reducing condition

Activity

Measured by its binding ability in a functional ELISA with Recombinant Human ACE-2 Fc Chimera (Catalog # 10544-ZN).

Scientific Data Images for Recombinant BatCoV RaTG13 Spike (GCN4-IZ) His Protein, CF

Recombinant BatCoV RaTG13 Spike (GCN4-IZ) His-tag Protein Binding Activity.

Recombinant BatCoV RaTG13 Spike (GCN4-IZ) His-tag (Catalog # 10688-CV) binds Recombinant Human ACE-2 Fc Chimera (10544-ZN) in a functional ELISA.

Recombinant BatCoV RaTG13 Spike (GCN4-IZ) His-tag Protein SDS-PAGE.

2 μg/lane of Recombinant BatCoV RaTG13 Spike (GCN4-IZ) His-tag (Catalog # 10688-CV) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 145-165 kDa.

Formulation, Preparation, and Storage

10688-CV
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Spike

SARS-CoV-2, which causes the global pandemic coronavirus disease 2019 (Covid-19), belongs to a family of viruses known as coronaviruses that also include MERS-CoV and SARS-CoV-1. The bat coronavirus RaTG13 was identified as the closest known relative and likely origin for SARS-Cov-2, though how SARS-CoV-2 evolved to infect humans remains unclear. Coronaviruses are commonly comprised of four structural proteins: Spike protein (S), Envelope protein (E), Membrane protein (M) and Nucleocapsid protein (N) (1). The S protein is a homotrimeric glycoprotein, with each ~180-kDa monomer consisting of two subunits, S1 and S2, and it mediates membrane fusion and viral entry (2). As with most coronaviruses, proteolytic cleavage of the S protein into two distinct peptides, S1 and S2 subunits, is required for activation. The S1 subunit is focused on attachment of the protein to the host receptor while the S2 subunit is involved with cell fusion (3-5). A metallopeptidase, angiotensin-converting enzyme 2 (ACE2), has been identified as a functional receptor for SARS-CoV-2 through interaction with a receptor binding domain (RBD) located at the C-terminus of S1 subunit (6, 7). The S protein of RaTG13 shares 76% and 97% amino acid (aa) sequence identity with the S protein of SARS-CoV-1 and SARS-CoV-2, respectively. Despite high aa identity to SARS-CoV-2, five of the six key amino acids involved in ACE2 binding are different in bat RaTG13, leading to >1000 fold weaker binding to human ACE2 (8, 9). Polyclonal antibodies to the RBD of the SARS-CoV-2 S1 subunit have been shown to inhibit interaction with the ACE2 receptor, confirming RBD as an attractive target for vaccinations or antiviral therapy (10). There is also promising work showing that the RBD may be used to detect presence of neutralizing antibodies present in a patient's bloodstream, consistent with developed immunity after exposure to the SARS‑CoV‑2 virus (11).

References

  1. Wu, F. et al. (2020) Nature 579:265.
  2. Tortorici, M.A. and D. Veesler (2019) Adv. Virus Res. 105:93.
  3. Bosch, B.J. et al. (2003) J. Virol. 77:8801.
  4. Belouzard, S. et al. (2009) Proc. Natl. Acad. Sci. 106:5871.
  5. Millet, J.K. and G. R. Whittaker (2015) Virus Res. 202:120.
  6. Li, W. et al. (2003) Nature 426:450.
  7. Wong, S.K. et al. (2004) J. Biol. Chem. 279:3197.
  8. Malayia, J. et al. (2020) J Med. Virol. https://doi.org/10.1002/jmv.26261.
  9. Wrobel, A.G. et al. (2020) Nat. struct. Mol. Biol. https://doi.org/10.1038/s41594-020-0468-7.
  10. Tai, W. et al. (2020) Cell. Mol. Immunol. https://doi.org/10.1016/j.it.2020.03.007.
  11. Okba, N. M. A. et al. (2020) Emerg. Infect. Dis. https://doi.org/10.3201/eid2607.200841.

Long Name

Spike Protein

Alternate Names

S Protein

Entrez Gene IDs

918758 (HCoV-229E); 2943499 (HCoV-NL63); 39105218 (HCoV-OC43); 37616432 (MERS-CoV); 1489668 (SARS-CoV); 43740568 (SARS-CoV-2)

Gene Symbol

S

UniProt

QHR63300.2

Additional Spike Products

Product Documents for Recombinant BatCoV RaTG13 Spike (GCN4-IZ) His Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant BatCoV RaTG13 Spike (GCN4-IZ) His Protein, CF

For research use only

Related Research Areas

Infectious Diseases

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