Recombinant BatCoV RaTG13 Spike (GCN4-IZ) His Protein, CF

Catalog # Availability Size / Price Qty
10688-CV-100
Recombinant BatCoV RaTG13 Spike (GCN4-IZ) His-tag Protein Binding Activity.
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Recombinant BatCoV RaTG13 Spike (GCN4-IZ) His Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA with Recombinant Human ACE-2 Fc Chimera (Catalog # 10544-ZN).
Source
Human embryonic kidney cell, HEK293-derived batcov ratg13 Spike protein
BatCoV RaTG13 Spike
(Val16-Pro1209)
Accession # QHR63300.2
GCN4-IZHHHHHH
N-terminusC-terminus
Accession #
N-terminal Sequence
Analysis
Val16
Predicted Molecular Mass
138 kDa
SDS-PAGE
140-160 kDa, under reducing condition

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10688-CV

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Images

Binding Activity Recombinant BatCoV RaTG13 Spike (GCN4-IZ) His-tag Protein Binding Activity. View Larger

Recombinant BatCoV RaTG13 Spike (GCN4-IZ) His-tag (Catalog # 10688-CV) binds Recombinant Human ACE-2 Fc Chimera (10544-ZN) in a functional ELISA.

SDS-PAGE Recombinant BatCoV RaTG13 Spike (GCN4-IZ) His-tag Protein SDS-PAGE. View Larger

2 μg/lane of Recombinant BatCoV RaTG13 Spike (GCN4-IZ) His-tag (Catalog # 10688-CV) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 145-165 kDa.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Spike

SARS-CoV-2, which causes the global pandemic coronavirus disease 2019 (Covid-19), belongs to a family of viruses known as coronaviruses that also include MERS-CoV and SARS-CoV-1. The bat coronavirus RaTG13 was identified as the closest known relative and likely origin for SARS-Cov-2, though how SARS-CoV-2 evolved to infect humans remains unclear. Coronaviruses are commonly comprised of four structural proteins: Spike protein (S), Envelope protein (E), Membrane protein (M) and Nucleocapsid protein (N) (1). The S protein is a homotrimeric glycoprotein, with each ~180-kDa monomer consisting of two subunits, S1 and S2, and it mediates membrane fusion and viral entry (2). As with most coronaviruses, proteolytic cleavage of the S protein into two distinct peptides, S1 and S2 subunits, is required for activation. The S1 subunit is focused on attachment of the protein to the host receptor while the S2 subunit is involved with cell fusion (3-5). A metallopeptidase, angiotensin-converting enzyme 2 (ACE2), has been identified as a functional receptor for SARS-CoV-2 through interaction with a receptor binding domain (RBD) located at the C-terminus of S1 subunit (6, 7). The S protein of RaTG13 shares 76% and 97% amino acid (aa) sequence identity with the S protein of SARS-CoV-1 and SARS-CoV-2, respectively. Despite high aa identity to SARS-CoV-2, five of the six key amino acids involved in ACE2 binding are different in bat RaTG13, leading to >1000 fold weaker binding to human ACE2 (8, 9). Polyclonal antibodies to the RBD of the SARS-CoV-2 S1 subunit have been shown to inhibit interaction with the ACE2 receptor, confirming RBD as an attractive target for vaccinations or antiviral therapy (10). There is also promising work showing that the RBD may be used to detect presence of neutralizing antibodies present in a patient's bloodstream, consistent with developed immunity after exposure to the SARS‑CoV‑2 virus (11).

References
  1. Wu, F. et al. (2020) Nature 579:265.
  2. Tortorici, M.A. and D. Veesler (2019) Adv. Virus Res. 105:93.
  3. Bosch, B.J. et al. (2003) J. Virol. 77:8801.
  4. Belouzard, S. et al. (2009) Proc. Natl. Acad. Sci. 106:5871.
  5. Millet, J.K. and G. R. Whittaker (2015) Virus Res. 202:120.
  6. Li, W. et al. (2003) Nature 426:450.
  7. Wong, S.K. et al. (2004) J. Biol. Chem. 279:3197.
  8. Malayia, J. et al. (2020) J Med. Virol. https://doi.org/10.1002/jmv.26261.
  9. Wrobel, A.G. et al. (2020) Nat. struct. Mol. Biol. https://doi.org/10.1038/s41594-020-0468-7.
  10. Tai, W. et al. (2020) Cell. Mol. Immunol. https://doi.org/10.1016/j.it.2020.03.007.
  11. Okba, N. M. A. et al. (2020) Emerg. Infect. Dis. https://doi.org/10.3201/eid2607.200841.
Long Name
Spike Protein
Entrez Gene IDs
918758 (HCoV-229E); 2943499 (HCoV-NL63); 39105218 (HCoV-OC43); 37616432 (MERS-CoV); 1489668 (SARS-CoV); 43740568 (SARS-CoV-2)
Alternate Names
2019-nCoV S Protein; 2019-nCoV Spike; COVID-19 Spike; E2; Human coronavirus spike glycoprotein; Peplomer protein; S glycoprotein; S Protein; SARS-COV-2 S protein; SARS-COV-2 Spike glycoprotein; SARSCOV2 Spike protein; SARS-CoV-2; Severe Acute Respiratory Syndrome Coronavirus 2 Spike Protein; Spike glycoprotein; Spike; surface glycoprotein

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