Recombinant Cynomolgus B7-H6 Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
8984-B7-050
8984-B7-01M
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Recombinant Cynomolgus B7-H6 Fc Chimera Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to induce IFN-gamma secretion by NK‑92 human natural killer lymphoma cells. Brandt, C.S. et al. (2009) J. Exp. Med. 206:1495. The ED50 for this effect is 0.5-2.5 μg/mL.
Source
Human embryonic kidney cell, HEK293-derived cynomolgus monkey B7-H6 protein
Cynomolgus B7-H6
(Asp25-Ser262)
Accession # XP_005578557
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminusC-terminus
Accession #
N-terminal Sequence
Analysis
Asp25
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
53 kDa
SDS-PAGE
70-81 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

8984-B7

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Reconstitution Calculator

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Background: B7-H6

B7-H6 is a 55 kDa glycosylated member of the B7 family of immune costimulatory proteins (1, 2). Mature B7-H6 consists of an extracellular domain (ECD) that with one Ig-like V domain and one Ig-like C1 domain, a transmembrane segment, and a cytoplasmic domain that contains one ITIM, one SH2, and one SH3 motif (3). Both of the Ig-like domains carry N-linked glycosylation (4). Within the ECD, cynomolgous B7-H6 shares 81%, 81%, 77% and 80% amino acid sequence identity with chimpanzee, orangutan, gibbon, and human B7-H6, respectively. Orthologs in mouse and rat have not been identified. The Ig-like V domain mediates 1:1 stoichiometric binding of B7-H6 to NKp30 expressed on NK cells (4, 5). It does not show binding to NKp44, NKp46, or NKG2D (3, 6). Ligation of NKp30 by B7-H6 induces NK cell activation and target cell cytolysis (3). B7-H6 is expressed on a wide range of hematopoietic, carcinoma, and melanoma tumor cells, which is consistent with the detection of NKp30 binding sites on many tumors (3, 7). B7-H6 is up-regulated on inflammatory monocytes and neutrophils, and a 30 kDa soluble fragment can be released by ADAM-10 or ADAM-17 mediated shedding (8, 9). The expression of B7-H6 on tumor cells correlates with cancer progression and patient's survival in human ovarian cancer (10).

References
  1. Zou, W. and L. Chen (2008) Nat. Rev. Immunol. 8:467.
  2. Bour-Jordan, H. et al. (2011) Immunol. Rev. 241:180.
  3. Brandt, C.S. et al. (2009) J. Exp. Med. 206:1495.
  4. Li, Y. et al. (2011) J. Exp. Med. 208:703.
  5. Joyce, M.G. et al. (2011) Proc. Natl. Acad. Sci. USA 108:6223.
  6. Arnon, T.I. et al. (2006) Semin. Cancer Biol. 16:348.
  7. Byrd, A. et al. (2007) PLoS ONE 2:e1339.
  8. Matta, J. et al. (2013) Blood 122:394.
  9. Schlecker, E. et al. (2014) Cancer Res. 74:3429.
  10. Zhou, Y. et al. (2015) Int. J. Clin. Exp. Pathol. 8:9428.
Long Name
B7 Homolog 6
Entrez Gene IDs
374383 (Human); 102121659 (Cynomolgus Monkey)
Alternate Names
B7 homolog 6; B7H6; B7-H6; DKFZp686I21167; DKFZp686O24166; NCR3LG1

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