Recombinant Cynomolgus CD200 R1 His-tag Protein, CF

Catalog # Availability Size / Price Qty
10055-CD-050
Recombinant Cynomolgus CD200 R1 His-tag Protein, CF Binding Activity
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Recombinant Cynomolgus CD200 R1 His-tag Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Cynomolgus Monkey CD200 R1 is coated at 0.5 μg/mL (100 μL/well), the concentration of Recombinant Human CD200 Fc Chimera (Catalog # 2724-CD) that produces 50% optimal binding response is 0.02‑0.12 μg/mL
Source
Human embryonic kidney cell, HEK293-derived cynomolgus monkey CD200 R1 protein
Ala27-Leu267, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Ala27
Predicted Molecular Mass
28 kDa
SDS-PAGE
60-67 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10055-CD

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 1 mg/mL in water.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Data Images

Binding Activity Binding Activity View Larger

When Recombinant Cynomolgus Monkey CD200 R1 (Catalog # 10055-CD) is coated at 0.5 μg/mL, 100 μL/well, Recombinant Human CD200 Fc Chimera (Catalog # 2724-CD) binds with an ED50 of 0.02‑0.12 μg/mL.

SDS-PAGE SDS-PAGE View Larger

2 μg/lane of Recombinant Cynomolgus Monkey CD200 R1 was resolved with SDS-PAGE underreducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Bluestaining, showing bands at 60-67 kDa.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CD200 R1

CD200 R1, also known as OX-2 receptor, is a 90 kDa transmembrane protein in the immunoglobulin superfamily and is important in the regulation of myeloid cell activity (1-3). The cynomolgus CD200 R1 cDNA encodes a 241 amino acid (aa) extracellular domain (ECD) and 61 aa cytoplasmic tail. The ECD is composed of one Ig-like V‑type domain and one Ig-like C2-type domain (4). Within the ECD, cynomolgus CD200 R1 shares 91%, 54%, and 57% aa sequence identity with human, mouse, and rat CD200 R1, respectively. Alternate splicing of the human CD200 R1 mRNA generates four isoforms, two of which are truncated in the Ig-C2 domain and are likely secreted (5). CD200 R1 expression is restricted primarily to mast cells, basophils, macrophages, and dendritic cells (6-8), while its ligand, CD200, is widely distributed (9). Disruption of this receptor-ligand system by knockout of the CD200 gene in mice leads to increased macrophage number and activation and predisposition to autoimmune disorders (10). Association of CD200 with CD200 R1 takes place between their respective N-terminal Ig-like domains (11). CD200 R1 propagates inhibitory signals despite lacking a cytoplasmic ITIM (immunoreceptor tyrosine-based inhibitory motif) (7, 8, 12, 13). CD200R1 signaling inhibits the expression of proinflammatory molecules including TNFs, IFNs, and inducible nitric oxide synthase in response to selected stimuli, which implicate that CD200/CD200R1 inhibitory signaling pathway plays a prominent role in limiting inflammation in a wide range of inflammatory diseases (14). Furthermore, the CD200/CD200R inhibitory signaling constitutes one of the most suitable endogenous immunoregulatory molecule candidate to restore the immune suppressive status of the CNS altered in chronic neuroinflammatory situations (15).

References
  1. Rosenblum, M.D. et al. (2006) J. Dermatol. Sci. 41:165.
  2. Gorczynski, R.M. (2005) Curr. Opin. Invest. Drugs 6:483.
  3. Barclay, A.N. et al. (2002) Trends Immunol. 23:285.
  4. Wright, G.J. et al. (2003) J. Immunol. 171:3034.
  5. Vieites, J.M. et al. (2003) Gene. Jun. 5; 311:99.
  6. Shiratori, I. et al. (2005) J. Immunol. 175:4441.
  7. Cherwinski, H.M. et al. (2005) J. Immunol. 174:1348.
  8. Fallarino, F. et al. (2004) J. Immunol. 173:3748.
  9. Wright, G.J. et al. (2001) Immunology 102:173.
  10. Hoek, R.M. et al. (2000) Science 290:1768.
  11. Hatherley, D. and A.N. Barclay (2004) Eur. J. Immunol. 34:1688.
  12. Jenmalm, M.C. et al. (2006) J. Immunol. 176:191.
  13. Zhang, S. et al. (2004) J. Immunol. 173:6786.
  14. Vaine, C.A. et al. (2014) Adv Immunol.121: 191.
  15. Hernangómez, M. et al. (2014) Curr Pharm Des. 20:4707.
Long Name
CD200 Receptor 1
Entrez Gene IDs
131450 (Human); 57781 (Mouse); 102139962 (Cynomolgus Monkey)
Alternate Names
CD200 R1; CD200 receptor 1; CD200R1; CD200RMOX2Rcell surface glycoprotein CD200 receptor 1; Cell surface glycoprotein OX2 receptor 1; cell surface glycoprotein receptor CD200; CRTR2; HCRTR2; MOX2 receptor; MOX2R; OX2RCD200 cell surface glycoprotein receptor

FAQs

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Primary Antibodies

His Tag Antibody

WB,Simple Western,Flow,AP,CyTOF-ready

Protein Purification Kits

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