Recombinant Cynomolgus Fc gamma RIIA/CD32a Protein, CF
Recombinant Cynomolgus Fc gamma RIIA/CD32a Protein, CF Summary
Thr29-Ile211, with a C-terminal 6-His tag
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 200 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
When Recombinant Cynomolgus Monkey Fc gamma RIIA/CD32a (Catalog # 9240-CD) is immobilized on a His Tag Antibody coated plate, Biotinylated Human IgG binds with an ED50 of 0.75-3.75 μg/mL.
Background: Fc gamma RIIA/CD32a
Receptors for the Fc region of IgG (Fc gamma Rs) are members of the Ig superfamily that function in the activation or inhibition of immune responses such as degranulation, phagocytosis, ADCC (antibody-dependent cellular toxicity), cytokine release, and B cell proliferation (1, 2). The Fc gamma Rs have been divided into three classes based on close relationships in their extracellular domains; these groups are designated Fc gamma RI/CD64, Fc gamma RII/CD32, and Fc gamma RIII/CD16. CD32 proteins are encoded by three genes in human (Fc gamma RII A, B, and C), two in cynomolgus (Fc gamma RII A and B), and one in mouse (Fc gamma RIIB). Fc gamma RII A and C contain ITAM sequences, while Fc gamma RIIB contains an ITIM sequence in their cytoplasmic domains. The CD64 proteins are high affinity receptors (~10-8-10-9 M) capable of binding monomeric IgG, whereas the CD16 and CD32 proteins bind IgG with lower affinities (~10-6-10-7 M) and only recognize IgG aggregates surrounding multivalent antigens (1, 3). Mature cynomolgus Fc gamma RIIA consists of a 183 amino acid (aa) extracellular domain (ECD) with two Ig-like domains, a 23 aa transmembrane segment, and a 77 aa cytoplasmic domain. Within the ECD, cynomolgus Fc gamma RIIA shares 89% aa sequence identity with human Fc gamma RIIA. It binds cynomolgus and human IgG subclasses 1-4 as well as human Pentraxin-2, and C-Reactive Protein (4, 5). It delivers activating signals through its cytoplasmic ITAM sequence (6). Fc gamma RIIA signaling cooperates with Integrin alpha 2b beta 3 and the CD42b-GP1b-IX-V complex to promote platelet adhesion and activation (7, 8). Fc gamma RIIA is expressed on monocytes, monocytes, and platelets and additionally on cynomolgus granulocytes (1, 4).
- Qiao, J. et al. (2015) Immunol. Rev. 268:241.
- Ravetch, J. and S. Bolland (2001) Annu. Rev. Immunol. 19:275.
- Takai, T. (2002) Nature Rev. Immunol. 2:580.
- Warncke, M. et al. (2012) J. Immunol. 188:4405.
- Lu, J. et al. (2008) Nature 456:989.
- Rosenfeld, S.I. et al. (1985) J. Clin. Invest. 76:2317.
- Sullam, P.M. et al. (1998) J. Biol. Chem. 273:5331.
- Boylan, B. et al. (2008) Blood 112:2780.
No product specific FAQs exist for this product, however you mayView all Proteins and Enzyme FAQs
Reviews for Recombinant Cynomolgus Fc gamma RIIA/CD32a Protein, CF
There are currently no reviews for this product. Be the first to review Recombinant Cynomolgus Fc gamma RIIA/CD32a Protein, CF and earn rewards!
Have you used Recombinant Cynomolgus Fc gamma RIIA/CD32a Protein, CF?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image